Diversity and Efficiency of Rhizobia from a Revegetated Area and Hotspot-Phytophysiognomies Affected by Iron Mining as Indicators of Rehabilitation and Biotechnological Potential.

Autor: Freitas Costa P; Setor de Biologia, Microbiologia e Processos Biológicos do Solo, Soil Science Department, Universidade Federal de Lavras, Lavras, Minas Gerais, CEP 37200-900, Brazil., Oliveira Silva A; Setor de Biologia, Microbiologia e Processos Biológicos do Solo, Soil Science Department, Universidade Federal de Lavras, Lavras, Minas Gerais, CEP 37200-900, Brazil., Azarias Guimarães A; Setor de Biologia, Microbiologia e Processos Biológicos do Solo, Soil Science Department, Universidade Federal de Lavras, Lavras, Minas Gerais, CEP 37200-900, Brazil., Resende de Assis LL; Setor de Biologia, Microbiologia e Processos Biológicos do Solo, Soil Science Department, Universidade Federal de Lavras, Lavras, Minas Gerais, CEP 37200-900, Brazil., Rufini M; Setor de Biologia, Microbiologia e Processos Biológicos do Solo, Soil Science Department, Universidade Federal de Lavras, Lavras, Minas Gerais, CEP 37200-900, Brazil., de Paiva Barbosa L; Setor de Biologia, Microbiologia e Processos Biológicos do Solo, Soil Science Department, Universidade Federal de Lavras, Lavras, Minas Gerais, CEP 37200-900, Brazil.; IFMG - Instituto Federal de Ciência e Tecnologia de Minas Gerais, Campus, Ponte Nova, MG, Brazil., Soares de Carvalho T; Setor de Biologia, Microbiologia e Processos Biológicos do Solo, Soil Science Department, Universidade Federal de Lavras, Lavras, Minas Gerais, CEP 37200-900, Brazil., de Souza Moreira FM; Setor de Biologia, Microbiologia e Processos Biológicos do Solo, Soil Science Department, Universidade Federal de Lavras, Lavras, Minas Gerais, CEP 37200-900, Brazil. fmoreira@ufla.br.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2022 Dec 19; Vol. 80 (1), pp. 40. Date of Electronic Publication: 2022 Dec 19.
DOI: 10.1007/s00284-022-03104-z
Abstrakt: This study aimed to evaluate the resilience of phytophysiognomies under influence of iron mining by assessing the occurrence, diversity, and symbiotic efficiency of native communities of nitrogen-fixing bacteria that nodulate leguminous plants (rhizobia) in soils of an area revegetated with grass after iron mining activities and in the phytophysiognomies in adjacent areas (Canga, Atlantic Forest, Cerrado, and Eucalyptus-planted forest). Experiments for capturing rhizobia through two species of promiscuous plants, siratro (Macroptilium atropurpureum) and cowpea (Vigna unguiculata), were conducted in a greenhouse. The rhizobial strains isolated were characterized phenotypically, genetically (16S rRNA sequencing and BOX-PCR fingerprinting), and regard symbiotic efficiency of biological nitrogen fixation (BNF) compared to mineral nitrogen and reference strains. Cowpea captured a higher density of rhizobia than siratro did. However, most of the strains captured by siratro had greater symbiotic efficiency. The revegetated area proved to be the community most efficient in N 2 fixation and was also the most diverse, whereas Canga was the least diverse. For the two trap species, the predominant genus captured in the revegetated area and in the phytophysiognomies was Bradyrhizobium. The greater symbiotic efficiency and the high genetic diversity of the rhizobial community in the revegetated area indicate the effectiveness of the soil rehabilitation process. The revegetated area and the phytophysiognomies proved to harbor strains with high biotechnological potential. Results indicate that the high functional redundancy of this group of bacteria contributes to the resilience of these phytophysiognomies and the revegetated area.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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