Renoprotective effect of N-acetylcystein and vitamin E in bisphenol A-induced rat nephrotoxicity; Modulators of Nrf2/ NF-κB and ROS signaling pathway.
| Autor: | Abdelrazik E; Lecturer in Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine Mansoura University, Egypt. ahmedemanali@yahoo.com., Hassan HM; 2- Lecturer in Anatomy and Embrology Department, Faculty of Medicine Mansoura University, Egypt.. dr.hend88@yahoo.com., Abdallah Z; 3- Lecturer in Department of Physiology, Faculty of Medicine Mansoura University, Egypt. ZienabAbdallah@mans.edu.eg., Magdy A; 4- Lecturer in Department of Biochemistry ,Faculty of Medicine Mansoura University, Egypt. alshimaamagdy82@yahoo.com., Farrag EA; 5- Lecturer in Department of Pharmacology ,Faculty of Medicine Mansoura University, Egypt. dreman_abdo_2010@mans.edu.eg. |
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| Jazyk: | angličtina |
| Zdroj: | Acta bio-medica : Atenei Parmensis [Acta Biomed] 2022 Dec 16; Vol. 93 (6), pp. e2022301. Date of Electronic Publication: 2022 Dec 16. |
| DOI: | 10.23750/abm.v93i6.13732 |
| Abstrakt: | Background and Aim of the Work: Bisphenol A (BPA) is a chemical product that is widely used as a plastic precursor. It acts directly on the kidney mitochondria, causing renal dysfunction. N-acetylcysteine is effective in protecting the kidneys from chemical-induced damage. Vitamin E is an antioxidant that protects cells from the damaging effects of free radicals. The aim of this study is to further evaluate and compare NAC and vitamin E to oppose the nephrotoxicity caused by BPA. Research Design and Methods: Forty-two adult male rats were divided into 7 groups: control, BPA, NAC, vitamin E, BPA plus NAC, BPA plus vitamin E, and combined BPA, NAC and vitamin E. BPA, NAC, vitamin E were given orally at doses of 50 mg/kg, 200 mg/kg, and 1000 mg/kg respectively, for 5 weeks. Results: NAC and vitamin E groups showed improved kidney function tests and alleviated BPA-induced oxidative stress; increased GSH and decreased MDA, NO and iNOS levels. NAC and vitamin E significantly attenuated inflammation; decreased NF-κB and increased IL-4, and Nrf2, in addition there was alleviation of renal histopathology. To some extent, vitamin E administration showed significant improvement. Moreover, combined NAC and vitamin E treatment showed more significance than either NAC or vitamin E separate groups. Conclusions: This study determined the substantial protective effects of NAC and/or vitamin E in BPA-induced nephrotoxicity through modulation of Nrf2 with subsequent improvement of oxidative stress and inflammation. The alleviation was more significant in combined NAC and vitamin E treatment mainly through their synergistic effect on Nrf2. |
| Databáze: | MEDLINE |
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