Cluster analysis and geospatial mapping of antibiotic resistant Escherichia coli O157 in southwest Nigerian communities.

Autor: Akinduti AP; Microbiology Unit, Department of Biological Sciences, Covenant University, Ota, Nigeria., Ayodele O; Department of Medical Microbiology, University College Hospital, Ibadan, Nigeria., Motayo BO; Department of Pathology, Federal Medical Centre, Abeokuta, Nigeria., Obafemi YD; Microbiology Unit, Department of Biological Sciences, Covenant University, Ota, Nigeria., Isibor PO; Applied Biology and Biotechnology Unit, Department of Biological Sciences, Covenant University, Ota, Nigeria., Aboderin OW; Department of Pathology, Federal Medical Centre, Abeokuta, Nigeria.
Jazyk: angličtina
Zdroj: One health (Amsterdam, Netherlands) [One Health] 2022 Oct 15; Vol. 15, pp. 100447. Date of Electronic Publication: 2022 Oct 15 (Print Publication: 2022).
DOI: 10.1016/j.onehlt.2022.100447
Abstrakt: Geospatial spread and antibiotic-resistant relatedness of Escherichia coli O157, which are important virulent serotypes causing severe complications leading to high intestinal morbidity and occasional mortality in several communities in southwest Nigeria, were evaluated. Biotyped Escherichia coli strains ( n  = 508) from subjects with diarrhea and related intestinal infections, various domestic water sources and food animal products were evaluated for antibiotic resistance relatedness, conjugative activity, virulence factor and biofilm production. Antibiotic resistance of Escherichia coli O157 encoded with stx was mapped for geospatial spread. Detected stx-encoded Escherichia coli O157 (7.56%) of human strains were significantly higher compared to water and food animal strains ( p  = 0.001) with high conjugative and transformative activity (OR(95%CI) = 34.65(94.5); p  = 0.023). Water- Escherichia coli O157 reveal significant median resistance to ciprofloxacin, gentamycin ( p  < 0.05) and human diarrheagenic strains showed >60% resistance to doxycycline (MIC 50 8 μg/mL and MIC 90 128 μg/mL; p  = 0.018), tetracycline (MIC 50 4 μg/mL and MIC 90 64 μg/mL), ciprofloxacin (MIC 50 2 μg/mL and MIC 90 128 μg/mL) and gentamycin (MIC 50 4 μg/mL and MIC 90 256 μg/mL). Strains from human diarrhea, UTI, colitis, cattle, fish, sheep, ground waters, streams, and rivers characterized with biofilm, hemolysin, protease productions, R-plasmid (≈14.30kbp) and MARI (0.84) were highly related. Principal component analysis (score plot) revealed a significant association between resistant human diarrheic strains with cattle and poultry strains. A high population of heterogeneous stx -encoded diarrheagenic and colitis strains was predominant in urban settings spreading with food animal and water Escherichia coli O157 strains. Human diarrheagenic Escherichia coli O157 were highly related to antibiotic resistance and virulence pattern with water and animal products strains. Strategic interventions through the implementation of One Health approach and population-target antimicrobial stewardship are needed to mitigate the increasing intestinal morbidity and reduction of mortality impact. Regular application of spatial data on clonal dissemination is important for monitoring, surveillance of antimicrobial resistance and transmission of zoonotic food-borne Escherichia coli O157 pathogens.
Competing Interests: The authors have declared that no competing interests exist.
(© 2022 The Authors.)
Databáze: MEDLINE
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