GRIN lens applications for studying neurobiology of substance use disorder.

Autor:	Beacher NJ; Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, United States., Washington KA; Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, United States., Zhang Y; Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, United States., Li Y; Department of Zoology and Physiology, University of Wyoming, Laramie, WY, United States., Lin DT; Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, United States.
Jazyk:	angličtina
Zdroj:	Addiction neuroscience [Addict Neurosci] 2022 Dec; Vol. 4. Date of Electronic Publication: 2022 Nov 24.
DOI:	10.1016/j.addicn.2022.100049
Abstrakt:	Substance use disorder (SUD) is associated with severe health and social consequences. Continued drug use results in alterations of circuits within the mesolimbic dopamine system. It is critical to observe longitudinal impacts of SUD on neural activity in vivo to identify SUD predispositions, develop pharmaceuticals to prevent overdose, and help people suffering from SUD. However, implicated SUD associated areas are buried in deep brain which makes in vivo observation of neural activity challenging. The gradient index (GRIN) lens can probe these regions in mice and rats. In this short communications review, we will discuss how the GRIN lens can be coupled with other technologies such as miniaturized microscopes, fiberscopes, fMRI, and optogenetics to fully explore in vivo SUD research. Particularly, GRIN lens allows in vivo observation of deep brain regions implicated in SUD, differentiation of genetically distinct neurons, examination of individual cells longitudinally, correlation of neuronal patters with SUD behavior, and manipulation of neural circuits. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Databáze:	MEDLINE
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