Extracellular Matrix Microparticles Improve GelMA Bioink Resolution for 3D Bioprinting at Ambient Temperature.

Autor: Galliger Z; Biomedical Engineering Graduate Program, University of Minnesota, Minneapolis, MN., Vogt CD; Biomedical Engineering Graduate Program; Medical Scientist Training Program, University of Minnesota, 420 Delaware St. SE, Minneapolis, MN., Helms HR; Department of Pediatrics, Division of Blood and Marrow Transplantation & Cell Therapy, University of Minnesota, 420 Delaware St. SE, Minneapolis, MN., Panoskaltsis-Mortari A; Department of Pediatrics, Division of Blood and Marrow Transplantation & Cell Therapy; Department of Medicine, Division of Pulmonary, Allergy, Critical Care & Sleep, University of Minnesota, 420 Delaware St. SE., Minneapolis, MN.
Jazyk: angličtina
Zdroj: Macromolecular materials and engineering [Macromol Mater Eng] 2022 Oct; Vol. 307 (10). Date of Electronic Publication: 2022 Jul 21.
DOI: 10.1002/mame.202200196
Abstrakt: Introduction: Current bioinks for 3D bioprinting, such as gelatin-methacryloyl, are generally low viscosity fluids at room temperature, requiring specialized systems to create complex geometries.
Methods and Results: Adding decellularized extracellular matrix microparticles derived from porcine tracheal cartilage to gelatin-methacryloyl creates a yield stress fluid capable of forming self-supporting structures. This bioink blend performs similarly at 25°C to gelatin-methacryloyl alone at 15°C in linear resolution, print fidelity, and tensile mechanics.
Conclusion: This method lowers barriers to manufacturing complex tissue geometries and removes the need for cooling systems.
Competing Interests: Ethics Statement & Conflict of Interest Ethical approval of this work was not required. The authors declare no conflict of interest for this work.
Databáze: MEDLINE
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