Cryptosporulation in Kurthia spp. forces a rethinking of asporogenesis in Firmicutes.

Autor: Fatton M; Laboratory of Microbiology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland., Filippidou S; Laboratory of Microbiology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.; AstrobiologyOU, The Open University, Milton Keynes, UK., Junier T; Laboratory of Microbiology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.; Vital-IT group, Swiss Institute of Bioinformatics, Lausanne, Switzerland., Cailleau G; Laboratory of Microbiology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland., Berge M; Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland., Poppleton D; Unité de Biologie Moléculaire du Gène chez les Extrémophiles, Département de Microbiologie, Institut Pasteur, France., Blum TB; Biology and Chemistry, Laboratory of Biomolecular Research, Paul Scherrer Institute (PSI), Villigen, Switzerland., Kaminek M; Institute for Anatomy, University of Bern, Bern, Switzerland., Odriozola A; Institute for Anatomy, University of Bern, Bern, Switzerland., Blom J; Bioinformatics and Systems Biology, Justus-Liebig-University Giessen, Giessen, Germany., Johnson SL; Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA., Abrahams JP; Biology and Chemistry, Laboratory of Biomolecular Research, Paul Scherrer Institute (PSI), Villigen, Switzerland.; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.; Institute of Biology, Leiden University, Leiden, The Netherlands., Chain PS; Institute for Anatomy, University of Bern, Bern, Switzerland., Gribaldo S; Unité de Biologie Moléculaire du Gène chez les Extrémophiles, Département de Microbiologie, Institut Pasteur, France., Tocheva EI; Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada., Zuber B; Institute for Anatomy, University of Bern, Bern, Switzerland., Viollier PH; Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland., Junier P; Laboratory of Microbiology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2022 Dec; Vol. 24 (12), pp. 6320-6335. Date of Electronic Publication: 2022 Aug 17.
DOI: 10.1111/1462-2920.16145
Abstrakt: Endosporulation is a complex morphophysiological process resulting in a more resistant cellular structure that is produced within the mother cell and is called endospore. Endosporulation evolved in the common ancestor of Firmicutes, but it is lost in descendant lineages classified as asporogenic. While Kurthia spp. is considered to comprise only asporogenic species, we show here that strain 11kri321, which was isolated from an oligotrophic geothermal reservoir, produces phase-bright spore-like structures. Phylogenomics of strain 11kri321 and other Kurthia strains reveals little similarity to genetic determinants of sporulation known from endosporulating Bacilli. However, morphological hallmarks of endosporulation were observed in two of the four Kurthia strains tested, resulting in spore-like structures (cryptospores). In contrast to classic endospores, these cryptospores did not protect against heat or UV damage and successive sub-culturing led to the loss of the cryptosporulating phenotype. Our findings imply that a cryptosporulation phenotype may have been prevalent and subsequently lost by laboratory culturing in other Firmicutes currently considered as asporogenic. Cryptosporulation might thus represent an ancestral but unstable and adaptive developmental state in Firmicutes that is under selection under harsh environmental conditions.
(© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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