Nanovesicle and extracellular polymeric substance synthesis from the remediation of heavy metal ions from soil.

Autor: Budamagunta V; Department of Genetic Engineering, SRM Institute of Science and Technology, Chennai, 603203, India. Electronic address: vivekanandabudamagunta94@gmail.com., Shameem N; Department of Environmental Science, Cluster University Srinagar 190001, India. Electronic address: nowsheen.nowsheri@gmail.com., Irusappan S; Department of Genetic Engineering, SRM Institute of Science and Technology, Chennai, 603203, India. Electronic address: balasivaraj00@gmail.com., Parray JA; Department of Environmental Science, HKM Government Degree College Eidgah, Jammu and Kashmir 190017, India. Electronic address: javid06@gmail.com., Thomas M; Department of Genetic Engineering, SRM Institute of Science and Technology, Chennai, 603203, India. Electronic address: merin4mann@gmail.com., Marimuthu S; K.S. Rangasamy College of Technology, Thiruchengode, India. Electronic address: msanthoshnano@gmail.com., Kirubakaran R; Department of Biotechnology, Vinayaka Mission's Kirupananda Variyar Engineering College, Vinayaka Mission's Research Foundation, Salem, India. Electronic address: rangasamykirubakaran@gmail.com., Arul Jothi KN; Department of Genetic Engineering, SRM Institute of Science and Technology, Chennai, 603203, India. Electronic address: aruljotn@srmist.edu.in., Sayyed RZ; Department of Microbiology, PSGVP Mandal's S I Patil Arts, G B Patel Science and STKV Sangh Commerce College, Shahada 425409, India. Electronic address: sayyedrz@gmail.com., Show PL; Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou 325035, China; Department of Sustainable Engineering, Saveetha School of Engineering, SIMATS, Chennai, 602105, India; Department of Chemical and Environmental Engineering, University of Nottingham, Malaysia, 43500 Semenyih, Selangor Darul Ehsan, Malaysia. Electronic address: showpauloke@gmail.com.
Jazyk: angličtina
Zdroj: Environmental research [Environ Res] 2023 Feb 15; Vol. 219, pp. 114997. Date of Electronic Publication: 2022 Dec 15.
DOI: 10.1016/j.envres.2022.114997
Abstrakt: Heavy metal toxicity affects aquatic plants and animals, disturbing biodiversity and ecological balance causing bioaccumulation of heavy metals. Industrialization and urbanization are inevitable in modern-day life, and control and detoxification methods need to be accorded to meet the hazardous environment. Microorganisms and plants have been widely used in the bioremediation of heavy metals. Sporosarcina pasteurii, a gram-positive bacterium that is widely known for its calcite precipitation property in bio-cementing applications has been explored in the study for its metal tolerance ability for the first time. S. pasteurii SRMNP1 (KF214757) can tolerate silver stress to form nanoparticles and can remediate multiple heavy metals to promote the growth of various plants. This astounding property of the isolate warranted extensive examinations to comprehend the physiological changes during an external heavy metal stress condition. The present study aimed to understand various physiological responses occurring in S. pasteuriiSRMNP1 during the metal tolerance phenomenon using electron microscopy. The isolate was subjected to heavy metal stress, and a transmission electron microscope examination was used to analyze the physiological changes in bacteria to evade the metal stress. S. pasteurii SRMNP1 was tolerant against a wide range of heavy metal ions and can withstand a broad pH range (5-9). Transmission Electron Microscopy (TEM) examination of S. pasteurii SRMNP1 followed by 5 mM nickel sulfate treatment revealed the presence of nanovesicles encapsulating nanosized particles in intra and extracellular spaces. This suggests that the bacteria evade the metal stress by converting the metal ions into nanosized particles and encapsulating them within nanovesicles to efflux them through the vesicle budding mechanism. Moreover, the TEM images revealed an excessive secretion of extracellular polymeric substances by the strain to discharge the metal particles outside the bacterial system. S. pasteurii can be foreseen as an effective bioremediation agent with the potential to produce nanosized particles, nanovesicles, and extracellular polymeric substances. This study provides physiological evidence that, besides calcium precipitation applications, S. pasteurii can further be explored for its multidimensional roles in the fields of drug delivery and environmental engineering.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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