Cave Thiovulum (Candidatus Thiovulum stygium) differs metabolically and genomically from marine species.

Autor: Bizic M; Leibniz Institute for Freshwater Ecology and Inland Fisheries, IGB, Dep 3, Plankton and Microbial Ecology, Zur Alte Fischerhütte 2, OT Neuglobsow, 16775, Stechlin, Germany. mina.bizic@igb-berlin.de.; Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), Berlin, Germany. mina.bizic@igb-berlin.de., Brad T; 'Emil Racoviţă' Institute of Speleology, Clinicilor 5-7, 400006, Cluj-Napoca Romania, Romania. traian.brad@academia-cj.ro., Ionescu D; Leibniz Institute for Freshwater Ecology and Inland Fisheries, IGB, Dep 3, Plankton and Microbial Ecology, Zur Alte Fischerhütte 2, OT Neuglobsow, 16775, Stechlin, Germany. danny.ionescu@igb-berlin.de.; Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), Berlin, Germany. danny.ionescu@igb-berlin.de., Barbu-Tudoran L; Center for Electron Microscopy, 'Babeș-Bolyai' University, Clinicilor 5, 400006, Cluj-Napoca, Romania., Zoccarato L; Leibniz Institute for Freshwater Ecology and Inland Fisheries, IGB, Dep 3, Plankton and Microbial Ecology, Zur Alte Fischerhütte 2, OT Neuglobsow, 16775, Stechlin, Germany.; Institute of Computational Biology, University of Natural Resources and Life Sciences, Gregor-Mendel-Straße 3, 31180, Vienna, Austria., Aerts JW; Department of Molecular Cell Physiology, Faculty of Earth and Life sciences, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands., Contarini PE; Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum National d'Histoire Naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, 97110, Pointe-à-Pitre, France.; Laboratory for Research in Complex Systems, Menlo Park, CA, USA., Gros O; Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum National d'Histoire Naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, 97110, Pointe-à-Pitre, France., Volland JM; Laboratory for Research in Complex Systems, Menlo Park, CA, USA.; Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 94720, Berkeley, CA, USA., Popa R; River Road Research, 62 Leslie St, Buffalo, NY, 1421, USA., Ody J; Evolutionary Biology and Ecology, Université libre de Bruxelles (ULB), C.P. 160/12, Avenue F.D. Roosevelt 50, 1050, Brussels, Belgium., Vellone D; Vermont Integrative Genomics Lab, University of Vermont Cancer Center, Health Science Research Facility, Burlington, Vermont, VT, 05405, USA., Flot JF; Evolutionary Biology and Ecology, Université libre de Bruxelles (ULB), C.P. 160/12, Avenue F.D. Roosevelt 50, 1050, Brussels, Belgium.; Interuniversity Institute of Bioinformatics in Brussels-(IB)², Brussels, Belgium., Tighe S; Vermont Integrative Genomics Lab, University of Vermont Cancer Center, Health Science Research Facility, Burlington, Vermont, VT, 05405, USA., Sarbu SM; 'Emil Racoviţă' Institute of Speleology, Frumoasă 31-B, 010986, Bucureşti, Romania.; Department of Biological Sciences, California State University, Chico, CA, 95929, USA.
Jazyk: angličtina
Zdroj: The ISME journal [ISME J] 2023 Mar; Vol. 17 (3), pp. 340-353. Date of Electronic Publication: 2022 Dec 17.
DOI: 10.1038/s41396-022-01350-4
Abstrakt: Thiovulum spp. (Campylobacterota) are large sulfur bacteria that form veil-like structures in aquatic environments. The sulfidic Movile Cave (Romania), sealed from the atmosphere for ~5 million years, has several aqueous chambers, some with low atmospheric O 2 (~7%). The cave's surface-water microbial community is dominated by bacteria we identified as Thiovulum. We show that this strain, and others from subsurface environments, are phylogenetically distinct from marine Thiovulum. We assembled a closed genome of the Movile strain and confirmed its metabolism using RNAseq. We compared the genome of this strain and one we assembled from public data from the sulfidic Frasassi caves to four marine genomes, including Candidatus Thiovulum karukerense and Ca. T. imperiosus, whose genomes we sequenced. Despite great spatial and temporal separation, the genomes of the Movile and Frasassi Thiovulum were highly similar, differing greatly from the very diverse marine strains. We concluded that cave Thiovulum represent a new species, named here Candidatus Thiovulum stygium. Based on their genomes, cave Thiovulum can switch between aerobic and anaerobic sulfide oxidation using O 2 and NO 3 - as electron acceptors, the latter likely via dissimilatory nitrate reduction to ammonia. Thus, Thiovulum is likely important to both S and N cycles in sulfidic caves. Electron microscopy analysis suggests that at least some of the short peritrichous structures typical of Thiovulum are type IV pili, for which genes were found in all strains. These pili may play a role in veil formation, by connecting adjacent cells, and in the motility of these exceptionally fast swimmers.
(© 2022. The Author(s).)
Databáze: MEDLINE
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