Extraction and Oxford Nanopore sequencing of genomic DNA from filamentous Actinobacteria.

Autor:	Alvarez-Arevalo M; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark (DTU), Kemitorvet, 2800 Kgs. Lyngby, Denmark., Sterndorff EB; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark (DTU), Kemitorvet, 2800 Kgs. Lyngby, Denmark., Faurdal D; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark (DTU), Kemitorvet, 2800 Kgs. Lyngby, Denmark., Jørgensen TS; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark (DTU), Kemitorvet, 2800 Kgs. Lyngby, Denmark. Electronic address: tuesparholt@gmail.com., Mourched AS; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark (DTU), Kemitorvet, 2800 Kgs. Lyngby, Denmark., Vuksanovic O; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark (DTU), Kemitorvet, 2800 Kgs. Lyngby, Denmark., Saha S; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark (DTU), Kemitorvet, 2800 Kgs. Lyngby, Denmark., Weber T; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark (DTU), Kemitorvet, 2800 Kgs. Lyngby, Denmark. Electronic address: tiwe@biosustain.dtu.dk.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2023 Mar 17; Vol. 4 (1), pp. 101955. Date of Electronic Publication: 2022 Dec 16.
DOI:	10.1016/j.xpro.2022.101955
Abstrakt:	Actinomycetota (Actinobacteria) is an ecologically and industrially important phylum which is challenging to extract pure high-molecular-weight (HMW) DNA from. This protocol provides a parallelized, cost-effective, and straightforward approach for consistently extracting pure HMW DNA using modified non-toxic commercial kits suitable for higher throughput applications. We further provide a workflow for sequencing and assembly of complete genomes using an optimized Oxford Nanopore rapid barcoding protocol and Illumina data error correction. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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