Hedgehog signaling regulates Wolffian duct development through the primary cilium†.

Autor:	Alves MBR; Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, CHU de Québec Research Center (CHUL)-Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle-Université Laval, Quebec City, QC, Canada., Girardet L; Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, CHU de Québec Research Center (CHUL)-Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle-Université Laval, Quebec City, QC, Canada., Augière C; Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, CHU de Québec Research Center (CHUL)-Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle-Université Laval, Quebec City, QC, Canada., Moon KH; Department of Anatomy, Yonsei University College of Medicine, Seoul, Republic of Korea., Lavoie-Ouellet C; Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, CHU de Québec Research Center (CHUL)-Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle-Université Laval, Quebec City, QC, Canada., Bernet A; Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, CHU de Québec Research Center (CHUL)-Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle-Université Laval, Quebec City, QC, Canada., Soulet D; Faculty of Pharmacy, Department of Neurosciences, CHU de Québec Research Center (CHUL)-Université Laval, Quebec City, QC, Canada., Calvo E; Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, CHU de Québec Research Center (CHUL)-Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle-Université Laval, Quebec City, QC, Canada., Teves ME; Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, USA., Beauparlant CJ; Computational Biology Laboratory Research Centre, Faculty of Medicine, Université Laval, Quebec City, QC, Canada., Droit A; Computational Biology Laboratory Research Centre, Faculty of Medicine, Université Laval, Quebec City, QC, Canada., Bastien A; Faculty of Agriculture and Food Sciences, Department of Animal Sciences-Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle-Université Laval, Quebec City, QC, Canada., Robert C; Faculty of Agriculture and Food Sciences, Department of Animal Sciences-Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle-Université Laval, Quebec City, QC, Canada., Bok J; Department of Anatomy, Yonsei University College of Medicine, Seoul, Republic of Korea., Hinton BT; Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, USA., Belleannée C; Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, CHU de Québec Research Center (CHUL)-Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle-Université Laval, Quebec City, QC, Canada.
Jazyk:	angličtina
Zdroj:	Biology of reproduction [Biol Reprod] 2023 Feb 13; Vol. 108 (2), pp. 241-257.
DOI:	10.1093/biolre/ioac210
Abstrakt:	Primary cilia play pivotal roles in embryonic patterning and organogenesis through transduction of the Hedgehog signaling pathway (Hh). Although mutations in Hh morphogens impair the development of the gonads and trigger male infertility, the contribution of Hh and primary cilia in the development of male reproductive ductules, including the epididymis, remains unknown. From a Pax2Cre; IFT88fl/fl knock-out mouse model, we found that primary cilia deletion is associated with imbalanced Hh signaling and morphometric changes in the Wolffian duct (WD), the embryonic precursor of the epididymis. Similar effects were observed following pharmacological blockade of primary cilia formation and Hh modulation on WD organotypic cultures. The expression of genes involved in extracellular matrix, mesenchymal-epithelial transition, canonical Hh and WD development was significantly altered after treatments. Altogether, we identified the primary cilia-dependent Hh signaling as a master regulator of genes involved in WD development. This provides new insights regarding the etiology of sexual differentiation and male infertility issues. (© The Author(s) 2022. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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