Intra-uterine diazepam exposure decreases the number of catecholaminergic and serotoninergic neurons of neonate rats.

Autor: da Silva Junior CA; Department of Applied Science, William & Mary, Williamsburg, VA, United States; Department of Animal Morphology and Physiology, FCAV - UNESP - São Paulo State University, Jaboticabal, SP, Brazil., Marques DA; Department of Pediatrics, Québec Heart and Lung Institute, Université Laval, Québec, QC, Canada., Patrone LGA; Department of Animal Morphology and Physiology, FCAV - UNESP - São Paulo State University, Jaboticabal, SP, Brazil., Biancardi V; Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada., Bícego KC; Department of Animal Morphology and Physiology, FCAV - UNESP - São Paulo State University, Jaboticabal, SP, Brazil., Gargaglioni LH; Department of Animal Morphology and Physiology, FCAV - UNESP - São Paulo State University, Jaboticabal, SP, Brazil. Electronic address: luciane.gargaglioni@unesp.br.
Jazyk: angličtina
Zdroj: Neuroscience letters [Neurosci Lett] 2023 Jan 31; Vol. 795, pp. 137014. Date of Electronic Publication: 2022 Dec 12.
DOI: 10.1016/j.neulet.2022.137014
Abstrakt: Benzodiazepines, such as diazepam (DZP), are used to treat anxiety disorders, and are prescribed to pregnant woman for therapeutic purposes. Concerns regarding their consequences on postnatal development rise as they cross the placenta and interact with the embryo. Occurrence of malformation and behavioral syndromes have been reported for different ages, but little is known about their effects on the brain after exposure during intrauterine life. Thus, we sought to evaluate the effects of intrauterine exposure to DZP on the number of brainstem's catecholaminergic and serotonergic neurons, implicated in respiratory control, in male and female rats on postnatal (P) day 12-13, using immunofluorescence labeling for tyrosine-hydroxylase (TH) and serotonin (5-HT). We observed a reduction in the number of catecholaminergic neurons for males and females. Special attention is given to the reduction in the density of neurons in the A6 region, involved in ventilatory responses to CO 2 . Interestingly, only males showed a reduction in the number of serotonergic neurons, while females were not affected. These findings suggest that in utero exposure to DZP results in deleterious neuroanatomical effects on P12-13 rats and raises a note of concern for women clinicians to make more informed choices about the use of anxiolytic treatments during gestation.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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