Sample preparation for lipid analysis of intra-articular adipose tissue by using matrix-assisted laser desorption/ionization imaging.

Autor: Haartmans MJJ; Maastricht MultiModal Molecular Imaging Institute (M4i), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands; Laboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Joint-Preserving Clinic, CAPHRI Care and Public Health Research Institute, Maastricht University Medical Center+, Maastricht, the Netherlands. Electronic address: m.haartmans@maastrichtuniversity.nl., Claes BSR; Maastricht MultiModal Molecular Imaging Institute (M4i), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands. Electronic address: britt.claes@maastrichtuniversity.nl., Emanuel KS; Laboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Joint-Preserving Clinic, CAPHRI Care and Public Health Research Institute, Maastricht University Medical Center+, Maastricht, the Netherlands; Amsterdam UMC, Amsterdam Movement Sciences, Department of Orthopedic Surgery and Sports Medicine, Amsterdam, the Netherlands. Electronic address: k.emanuel@maastrichtuniversity.nl., Tuijthof GJM; Faculty of Engineering Technology, Biomedical Device Design and Production Technology (BDDP), Twente University, Twente, the Netherlands. Electronic address: g.j.m.tuijthof@utwente.nl., Heeren RMA; Maastricht MultiModal Molecular Imaging Institute (M4i), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands. Electronic address: r.heeren@maastrichtuniversity.nl., Emans PJ; Laboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Joint-Preserving Clinic, CAPHRI Care and Public Health Research Institute, Maastricht University Medical Center+, Maastricht, the Netherlands. Electronic address: p.emans@mumc.nl., Cillero-Pastor B; Maastricht MultiModal Molecular Imaging Institute (M4i), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands; MERLN Institute for Technology-inspired Regenerative Medicine, Department of Cell Biology-Inspired Tissue Engineering (cBITE), Maastricht University, Maastricht, the Netherlands. Electronic address: b.cilleropastor@maastrichtuniversity.nl.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2023 Feb 01; Vol. 662, pp. 115018. Date of Electronic Publication: 2022 Dec 12.
DOI: 10.1016/j.ab.2022.115018
Abstrakt: Mass spectrometry imaging (MSI) is a powerful technique enabling the visualization of the spatial distribution of different molecules in tissue biopsies with different pathologies. Sample handling and preparing adipose tissue for MSI is challenging and prone to molecular delocalization due to tissue melting. In this work, we developed a method for matrix-assisted laser desorption/ionization (MALDI)-MSI to study lipids in human infrapatellar fat pad (IPFP), a biomarker source in musculoskeletal pathologies, while preserving molecular spatial distribution. Cryosectioning at 15 μm with a temperature below -30 °C, thaw-mounting, and sublimation, was demonstrated to preserve IPFP's heterogeneous appearance and spatial distribution of lipids.
Competing Interests: Declaration of competing interest MH, KE, GT, RH, PE, and BCP are supported by the Dutch Research Council (NWO) domain Applied and Engineering Sciences (P15-23). MH, BC, RH, and BCP are supported by the Dutch Province of Limburg through the LINK (Limburg Investeert in haar Kenniseconomie) program. PE and BCP are supported by the Dutch Arthritis Society. PE has got a speaker consultancy fee paid to institute by Episurf and Arthrex (support for attending meeting). PE is involved with participation on a Data Safety Monitoring Board or Advisory Board (paid to institute) by Expert panel for Atro-Medical and Advisory board of TETEC AG. PE is vice chair of the international registree committee for joint-preserving surgery of the ICRS (no payments). PE is shareholder in Chondropeptix and Avalanche Medical.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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