Molecular diagnostics for gastrointestinal helminths in equids: Past, present and future.

Autor: Ghafar A; Melbourne Veterinary School, The University of Melbourne, Werribee, Victoria, Australia., Abbas G; Melbourne Veterinary School, The University of Melbourne, Werribee, Victoria, Australia., Beasley A; School of Agriculture and Food Science, University of Queensland, Gatton, Queensland, Australia., Bauquier J; Melbourne Veterinary School, The University of Melbourne, Werribee, Victoria, Australia., Wilkes EJA; Racing Victoria, Flemington, Victoria, Australia., Jacobson C; Centre for Animal Production and Health, Murdoch University, Murdoch, Western Australia, Australia., McConnell E; Centre for Animal Production and Health, Murdoch University, Murdoch, Western Australia, Australia., El-Hage C; Melbourne Veterinary School, The University of Melbourne, Werribee, Victoria, Australia., Carrigan P; Scone Equine Hospital, Scone, New South Wales, Australia., Cudmore L; Scone Equine Hospital, Scone, New South Wales, Australia., Tennent-Brown B; Melbourne Veterinary School, The University of Melbourne, Werribee, Victoria, Australia., Hurley J; Swettenham Stud, Nagambie, Victoria, Australia., Nielsen MK; M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA., Gauci CG; Melbourne Veterinary School, The University of Melbourne, Werribee, Victoria, Australia., Beveridge I; Melbourne Veterinary School, The University of Melbourne, Werribee, Victoria, Australia., Hughes KJ; School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, New South Wales, Australia., Jabbar A; Melbourne Veterinary School, The University of Melbourne, Werribee, Victoria, Australia. Electronic address: jabbara@unimelb.edu.au.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2023 Jan; Vol. 313, pp. 109851. Date of Electronic Publication: 2022 Dec 05.
DOI: 10.1016/j.vetpar.2022.109851
Abstrakt: This review is aimed to (i) appraise the literature on the use of molecular techniques for the detection, quantification and differentiation of gastrointestinal helminths (GIH) of equids, (ii) identify the knowledge gaps and, (iii) discuss diagnostic prospects in equine parasitology. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines for systematic reviews, we retrieved 54 studies (horses: 50/54; donkeys and zebras: 4/54) from four databases. Polymerase chain reaction (PCR) was employed in all of the studies whereas PCR amplicons were sequenced in only 18 of them. Other techniques used (including modifications of PCR) were reverse line blot, quantitative (q)PCR, restriction fragment length polymorphism, nested-PCR, PCR-directed next-generation sequencing, Southern blotting, single strand conformation polymorphism, PCR-enzyme linked immunosorbent assay, matrix-assisted laser desorption/ionisation-time of flight and random amplification of polymorphic DNA. Most of the studies (53/54) used nuclear ribosomal RNA (including the internal transcribed spacers, intergenic spacer, 5.8 S, 18 S, 28 S and 12 S) as target loci while cytochrome c oxidase subunit 1 and random genomic regions were targeted in only three and one studies, respectively. Overall, to date, the majority of molecular studies have focused on the diagnosis and identification of GIHs of equids (i.e. species of Anoplocephala, Craterostomum, cyathostomins, Oesophagodontus, Parascaris, Strongylus, Strongyloides and Triodontophorus), with a recent shift towards investigations on anthelmintic resistance and the use of high-throughput nemabiome metabarcoding. With the increasing reports of anthelmintic resistance in equid GIHs, it is crucial to develop and apply techniques such as advanced metabarcoding for surveillance of parasite populations in order to gain detailed insights into their diversity and sustainable control. To the best of our knowledge, this is the first systematic review that evaluates molecular investigations published on the diagnosis and quantification of equid GIHs and provides useful insights into important knowledge gaps and future research directions in equid molecular parasitology.
Competing Interests: Declaration of Competing Interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Boehringer Ingelheim did not have any role in the design or content of this manuscript.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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