Testosterone replacement relieves ligature-induced periodontitis by mitigating inflammation, increasing pro-resolving markers and promoting angiogenesis in rats: A preclinical study.

Autor: Pelegrin ÁF; Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University - UNESP, 1680 Humaitá St., - Center, 14801-903, Araraquara, SP, Brazil. Electronic address: alvaro.f.pelegrin@unesp.br., de Paiva Gonçalves V; Department of Dentistry, Pontifical Catholic University of Minas Gerais, 500 Dom José Gaspar Avenue, - Coração Eucarístico, 30535-901 Belo Horizonte, MG, Brazil. Electronic address: viniciusodonto2007@hotmail.com., Carvalho JS; Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University - UNESP, 1680 Humaitá St., - Center, 14801-903, Araraquara, SP, Brazil. Electronic address: js.carvalho@unesp.br., Spolidorio DMP; Department of Physiology and Pathology, School of Dentistry, São Paulo State University - UNESP, 1680 Humaitá St., - Center, 14801-903, Araraquara, SP, Brazil. Electronic address: denise.mp.spolidorio@unesp.br., Spolidorio LC; Department of Physiology and Pathology, School of Dentistry, São Paulo State University - UNESP, 1680 Humaitá St., - Center, 14801-903, Araraquara, SP, Brazil. Electronic address: luis-carlos.spolidorio@unesp.br.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2023 Feb; Vol. 146, pp. 105605. Date of Electronic Publication: 2022 Dec 12.
DOI: 10.1016/j.archoralbio.2022.105605
Abstrakt: Objectives: This study aimed to evaluate the inflammatory profile as well as the resolution of inflammation in a ligature-induced periodontal inflammation in rats with depletion and/or supraphysiological testosterone replacement.
Design: Sixty male rats (Holtzman) were used in the present study. Study groups were created as following: (1) Sham (no testicle removal); (2) Orchiectomy (OCX), 3) OCX + Testosterone (OCX + T); (4) Sham + Ligature (SH + L); (5) OCX+L; and 6) OCX + T + L. The surgeries were performed on day 1, and testosterone was administered weekly since day 1. On day 15, a cotton ligature was placed around the lower first molars and maintained for 15 days. Morphological changes in periodontal tissues were determined by histopathological analysis. Immunohistochemistry (factor VIII) and immunoenzymatic assay were performed to evaluate angiogenesis process and (pro- and anti-) inflammatory markers, respectively.
Results: Ligature promoted a marked inflammatory gingival infiltrate and bone loss (P < 0.05). Supraphysiological testosterone treatment increased the percentage of blood vessels, extracellular matrix and fibroblasts in the presence and absence of periodontal inflammation (P < 0.05). A high dose of testosterone increased factor VIII+ blood vessels and IL-10 expression in inflamed gingival tissue, while PGE 2 , LXA 4 and MPO were reduced as a result of supraphysiological testosterone administration (P < 0.05).
Conclusions: These results, in our experimental model, suggest that supraphysiological testosterone treatment stimulated gingival tissue repair during ligature-induced periodontitis, and it seems to be related to an anti-inflammatory and pro-resolutive mechanism resulting by the modulatory effect on PGE 2 and IL-10 related to an enhanced angiogenesis.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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