An updated protocol for in vitro bovine embryo production.
| Autor: | Speckhart SL; School of Animal Sciences, Blacksburg, VA 24060, USA. Electronic address: slspeckhart@vt.edu., Wooldridge LK; School of Animal Sciences, Blacksburg, VA 24060, USA., Ealy AD; School of Animal Sciences, Blacksburg, VA 24060, USA. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2023 Mar 17; Vol. 4 (1), pp. 101924. Date of Electronic Publication: 2022 Dec 13. |
| DOI: | 10.1016/j.xpro.2022.101924 |
| Abstrakt: | Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a unique procedure for washing and moving oocytes and zygotes between their respective dishes using a cell strainer. A thorough troubleshooting section will help users optimize embryo development with cleavage and blastocyst rates exceeding 70% and 20%, respectively. For complete details on the use and execution of this protocol, please refer to Wooldridge and Ealy (2019). 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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