Metabarcoding of the Three Domains of Life in Aquatic Saline Ecosystems.
| Autor: | Melayah D; Université de Lyon, INSA Lyon, CNRS, UMR5240 Microbiologie Adaptation et Pathogénie, Villeurbanne, France., Bontemps Z; Université de Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR5557 Ecologie Microbienne, Villeurbanne, France., Bruto M; Université de Lyon, VetAgro Sup, Anses, UMR Mycoplasmoses Animales, Marcy l'Etoile, France., Nguyen A; Microsynth France, Vaulx-en-Velin, France., Oger P; Université de Lyon, INSA Lyon, CNRS, UMR5240 Microbiologie Adaptation et Pathogénie, Villeurbanne, France., Hugoni M; Université de Lyon, INSA Lyon, CNRS, UMR5240 Microbiologie Adaptation et Pathogénie, Villeurbanne, France. mylene.hugoni@univ-lyon1.fr.; Institut Universitaire de France (IUF), Paris, France. mylene.hugoni@univ-lyon1.fr. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2605, pp. 17-35. |
| DOI: | 10.1007/978-1-0716-2871-3_2 |
| Abstrakt: | High-throughput amplicon sequencing, known as metabarcoding, is a powerful technique to decipher exhaustive microbial diversity considering specific gene markers. While most of the studies investigating ecosystem functioning through microbial diversity targeted only one domain of life, either bacteria, or archaea or microeukaryotes, the remaining challenge in microbial ecology is to uncover the integrated view of microbial diversity occurring in ecosystems. Indeed, interactions occurring between the different microbial counterparts are now recognized having a great impact on stability and resilience of ecosystems. Here, we summarize protocols describing sampling, molecular, and simultaneous metabarcoding of bacteria, archaea, and microeukaryotes, as well as a bioinformatic pipeline allowing the study of exhaustive microbial diversity in natural aquatic saline samples. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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