Lecithin-based nanocapsule loading sucupira ( Pterodon emarginatus ) oil effects in experimental mucositis.

Autor: Di Miceli JFF; Department of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, Brazil., Andrade MER; Department of Clinical and Toxicological Analysis, Pharmacy Faculty, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, Brazil., Carvalho PLA; Department of Clinical and Toxicological Analysis, Pharmacy Faculty, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, Brazil., Santos EA; Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, Brazil., Faria Mota Oliveira AEM; Department of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, Brazil., Fernandes CP; Department of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, Brazil., Cruz RAS; Department of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, Brazil., Garrett R; Metabolomics Laboratory, Institute of Chemistry, Universidade Federal do Rio de Janeiro, Av. Pedro Calmon, 550, Rio de Janeiro 21941-598, RJ, Brazil., Mosqueira VCF; Department of Pharmacy - Pharmacy School, Universidade Federal de Ouro Preto, (UFOP), Morro do Cruzeiro, Ouro Preto 35400-000, MG, Brazil., Cassali GD; Department de General Pathology, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, Brazil., D'Haese C; Université Catholique de Louvain, Institute of Condensed Matter and Nanosciences, Bio & Soft Matter, 1348 Louvain-la-Neuve, Belgium., Nysten B; Université Catholique de Louvain, Institute of Condensed Matter and Nanosciences, Bio & Soft Matter, 1348 Louvain-la-Neuve, Belgium., Leite JIA; Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, Brazil., Cardoso VN; Department of Clinical and Toxicological Analysis, Pharmacy Faculty, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, Brazil., Araújo RS; Department of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, Brazil.
Jazyk: angličtina
Zdroj: Toxicology reports [Toxicol Rep] 2022 Jul 11; Vol. 9, pp. 1537-1547. Date of Electronic Publication: 2022 Jul 11 (Print Publication: 2022).
DOI: 10.1016/j.toxrep.2022.07.006
Abstrakt: Intestinal mucositis (IM) is a frequent adverse effect in anticancer therapy without standard treatment. The oil obtained from sucupira (Pterodon emarginatus) has anti-inflammatory properties, and the soybean lecithin reduces the intestinal toxicity of several xenobiotics. However, their water insolubility impairs the in vivo application. For this reason, we evaluated if the nanoencapsulation of sucupira oil (SO) in lecithin-based nanocapsules (SO-NC) could be a therapeutically effective system for the treatment of IM in murine cisplatin (CDDP)-induced intestinal mucositis model. SO was analyzed by LC-HRMS/MS and HPLC. SO-NC was prepared by nanoprecipitation and characterized using DLS, HPLC, and AFM. Mice body weight and food consumption were assessed daily during experimental mucositis induced by CDDP. The animals were euthanized, and intestinal permeability, inflammatory mediators, and intestinal histology were performed. SO-NC demonstrated adequate characteristics for oral administration as size under 300 nm, IP < 0.3, high EE, and spherical shape. In vitro cytotoxicity performed against RAW 264.7 cell lines resulted in cell viability above 80 % confirming the non-cytotoxic profile of SO (IC 50 268 µg/mL) and SO-NC (IC 50 118.5 µg/mL) up to 117.2 µg/mL. The untreated mice showed intestinal toxicity after i.p. of CDDP, principally weight loss, increased intestinal permeability, and MPO and TNF-α levels. Surprisingly, the administration of SO to CDDP-mucositis animals did not circumvent the CDDP effects and increased intestinal permeability. However, SO-NC proved efficient in mitigating the experimental intestinal mucositis by improving intestinal epithelium architecture, reducing intestinal permeability, and improving the MPO levels. In conclusion, SO-NC can positively impact intestinal mucositis by promoting mucosal recovery. This is a promising strategy for developing a new treatment for intestinal mucositis.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2022 The Authors.)
Databáze: MEDLINE
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