The Drosophila melanogaster attP40 docking site and derivatives are insertion mutations of msp-300.

Autor: van der Graaf K; Department of Biosciences, Program in Biochemistry and Cell Biology, Rice University, Houston, Texas, United States of America., Srivastav S; Department of Biosciences, Program in Biochemistry and Cell Biology, Rice University, Houston, Texas, United States of America., Singh P; Department of Biosciences, Program in Biochemistry and Cell Biology, Rice University, Houston, Texas, United States of America., McNew JA; Department of Biosciences, Program in Biochemistry and Cell Biology, Rice University, Houston, Texas, United States of America., Stern M; Department of Biosciences, Program in Biochemistry and Cell Biology, Rice University, Houston, Texas, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Dec 14; Vol. 17 (12), pp. e0278598. Date of Electronic Publication: 2022 Dec 14 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0278598
Abstrakt: The ɸC31 integrase system is widely used in Drosophila melanogaster to allow transgene targeting to specific loci. Over the years, flies bearing any of more than 100 attP docking sites have been constructed. One popular docking site, termed attP40, is located close to the Nesprin-1 orthologue msp-300 and lies upstream of certain msp-300 isoforms and within the first intron of others. Here we show that attP40 causes larval muscle nuclear clustering, which is a phenotype also conferred by msp-300 mutations. We also show that flies bearing insertions within attP40 can exhibit decreased msp-300 transcript levels in third instar larvae. Finally, chromosomes carrying certain "transgenic RNAi project" (TRiP) insertions into attP40 can confer pupal or adult inviability or infertility, or dominant nuclear clustering effects in certain genetic backgrounds. These phenotypes do not require transcription from the insertions within attP40. These results demonstrate that attP40 and insertion derivatives act as msp-300 insertional mutations. These findings should be considered when interpreting data from attP40-bearing flies.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2022 van der Graaf et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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