Gigapixel imaging with a novel multi-camera array microscope.

Autor:	Thomson EE; Department of Neurobiology, Duke School of Medicine, Durham, United States., Harfouche M; Ramona Optics Inc, Durham, United States., Kim K; Biomedical Engineering, Duke University, Durham, United States., Konda PC; Biomedical Engineering, Duke University, Durham, United States., Seitz CW; Department of Neurobiology, Duke School of Medicine, Durham, United States., Cooke C; Biomedical Engineering, Duke University, Durham, United States., Xu S; Biomedical Engineering, Duke University, Durham, United States., Jacobs WS; Department of Neurobiology, Duke School of Medicine, Durham, United States., Blazing R; Department of Neurobiology, Duke School of Medicine, Durham, United States., Chen Y; Department of Neurobiology, Duke School of Medicine, Durham, United States., Sharma S; Ramona Optics Inc, Durham, United States., Dunn TW; Biomedical Engineering, Duke University, Durham, United States., Park J; Ramona Optics Inc, Durham, United States., Horstmeyer RW; Ramona Optics Inc, Durham, United States.; Biomedical Engineering, Duke University, Durham, United States., Naumann EA; Department of Neurobiology, Duke School of Medicine, Durham, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2022 Dec 14; Vol. 11. Date of Electronic Publication: 2022 Dec 14.
DOI:	10.7554/eLife.74988
Abstrakt:	The dynamics of living organisms are organized across many spatial scales. However, current cost-effective imaging systems can measure only a subset of these scales at once. We have created a scalable multi-camera array microscope (MCAM) that enables comprehensive high-resolution recording from multiple spatial scales simultaneously, ranging from structures that approach the cellular scale to large-group behavioral dynamics. By collecting data from up to 96 cameras, we computationally generate gigapixel-scale images and movies with a field of view over hundreds of square centimeters at an optical resolution of 18 µm. This allows us to observe the behavior and fine anatomical features of numerous freely moving model organisms on multiple spatial scales, including larval zebrafish, fruit flies, nematodes, carpenter ants, and slime mold. Further, the MCAM architecture allows stereoscopic tracking of the z-position of organisms using the overlapping field of view from adjacent cameras. Overall, by removing the bottlenecks imposed by single-camera image acquisition systems, the MCAM provides a powerful platform for investigating detailed biological features and behavioral processes of small model organisms across a wide range of spatial scales. Competing Interests: ET, KK, PK, CS, CC, SX, WJ, RB, YC, TD, EN No competing interests declared, MH is scientific co-founder at Ramona Optics Inc which is commercializing and patenting the multi-camera array microscope, SS, JP was an employee at Ramona Optics Inc, which is commercializing and patenting the multi-camera array microscope, RH is a scientific co-founder at Ramona Optics Inc, which is commercializing and patenting the multi-camera array microscope (© 2022, Thomson et al.)
Databáze:	MEDLINE
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