[Comparative study of Wuhan-like and omicron-like variants of SARS-CoV-2 in experimental animal models].

Autor:	Leneva IA; Mechnikov Research Institute of Vaccines and Sera, Department of Virology., Smirnova DI; Mechnikov Research Institute of Vaccines and Sera, Department of Virology., Kartashova NP; Mechnikov Research Institute of Vaccines and Sera, Department of Virology., Gracheva AV; Mechnikov Research Institute of Vaccines and Sera, Department of Virology., Ivanina AV; Mechnikov Research Institute of Vaccines and Sera, Department of Virology., Glubokova EA; Mechnikov Research Institute of Vaccines and Sera, Department of Virology., Korchevaya ER; Mechnikov Research Institute of Vaccines and Sera, Department of Virology., Pancratov AA; Herzen Moscow Research Institute of Oncology of the Ministry of Health of Russia., Trunova GV; Herzen Moscow Research Institute of Oncology of the Ministry of Health of Russia., Khokhlova VA; Herzen Moscow Research Institute of Oncology of the Ministry of Health of Russia., Svitich OA; Mechnikov Research Institute of Vaccines and Sera, Department of Virology., Zverev VV; Mechnikov Research Institute of Vaccines and Sera, Department of Virology., Faizuloev EB; Mechnikov Research Institute of Vaccines and Sera, Department of Virology.
Jazyk:	ruština
Zdroj:	Voprosy virusologii [Vopr Virusol] 2022 Nov 19; Vol. 67 (5), pp. 439-449. Date of Electronic Publication: 2022 Nov 19.
DOI:	10.36233/0507-4088-135
Abstrakt:	Introduction: The variability of SARS-CoV-2 appeared to be higher than expected, the emergence of new variants raises concerns. The aim of the work was to compare the pathogenicity of the Wuhan and BA.1.1/Omicron variants in BALB/c mice and Syrian hamsters. Materials and Methods: The study used strains of SARS-CoV-2: Dubrovka phylogenetically close to Wuhan-Hu-1, and LIA phylogenetically close to Omicron, BALB/c mice, transgenic mice B6.Cg-Tg(K18-ACE2)2Prlmn/HEMI Hemizygous for Tg(K18-ACE2)2Prlmn, Syrian golden hamsters. Animals were infected intranasally, pathogenicity was estimated by a complex of clinical, pathomorphological and virological methods. Results: Comparative studies of SARS-CoV-2 Dubrovka and LIA strains on animal models demonstrated their heterogeneous pathogenicity. In parallel infection of BALB/c mice with Dubrovka and LIA variants, the infection proceeded without serious clinical signs and lung damage. Infection with the LIA strain resulted to a systemic disease with a high concentration of viral RNA in the lungs and brain tissues of animals. The presence of viral RNA in mice infected with the Dubrovka strain was transient and undetectable in the lungs by day 7 post-infection. Unlike the mouse model, in hamsters, the Dubrovka strain had a greater pathogenicity than the LIA strain. In hamsters infected with the Dubrovka strain lung lesions were more significant, and the virus spread through organs, in particular in brain tissue, was observed. In hamsters infected with the LIA strain virus was not detected in brain tissue. Conclusion: The study of various variants of SARS-CoV-2 in species initially unsusceptible to SARS-CoV-2 infection is important for monitoring zoonotic reservoirs that increase the risk of spread of new variants in humans.
Databáze:	MEDLINE
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