Biodistribution and Targeted Antitumor Effects of Trastuzumab-Modified Gold Nanorods in Mice with Gastric Cancer.

Autor:	Yi T; Department of Ultrasound, Affiliated Union Hospital of Fujian Medical University, Fuzhou, China., Hongjiao C; Fisheries College of Jimei University, Xiamen, China., Minling Z; Department of Ultrasound, Affiliated Union Hospital of Fujian Medical University, Fuzhou, China., Xin Y; Department of Pharmacy, Affiliated Union Hospital of Fujian Medical University, Fuzhou, China., Qingfu Q; Department of Ultrasound, Affiliated Union Hospital of Fujian Medical University, Fuzhou, China., Zhixin C; Fujian College Association Instrumental Analysis Center, Fuzhou University, Fuzhou, China., Jing Y; Department of Pharmacy, Affiliated Union Hospital of Fujian Medical University, Fuzhou, China., Zhikui C; Department of Ultrasound, Affiliated Union Hospital of Fujian Medical University, Fuzhou, China.
Jazyk:	angličtina
Zdroj:	Current drug delivery [Curr Drug Deliv] 2024; Vol. 21 (3), pp. 421-430.
DOI:	10.2174/1567201820666221212125325
Abstrakt:	Background and Objectives: Targeted drug is often engulfed and cleared by the reticuloendothelial system in vivo , resulting in reduced treatment efficacy. This study aimed to explore the biodistribution and HER-2-targeted antitumor effects of trastuzumab-modified gold nanorods (Tra-AuNRs) in a gastric cancer animal model. Methods: Gold nanorods were synthesized using a seed-mediated growth method, and then subjected to trastuzumab-targeted modification. Elemental analysis, Fourier transform infrared spectroscopy, and Xray photoelectron spectroscopy were performed; UV-visible absorption peak, photothermal effects, morphology, and size distribution of Tra-AuNRs were characterized. The targeted killing effect of Tra- AuNRs on gastric cancer cells was assessed in vitro . Tra-AuNRs were injected intravenously and intratumorally into gastric cancer-bearing nude mice in vivo and their distribution was detected. Tumor growth inhibition rate and tumor apoptosis-related protein expression were compared between groups. Results: Tra-AuNRs presented a relatively uniform morphology with an average particle size of 59.9 nm and a longitudinal plasmon resonance absorption peak of 790 nm. The targeted killing rate of gastric cancer cells in vitro by Tra-AuNRs was 87.9%. After intravenous injection, Tra-AuNRs were mainly distributed in the liver, tumor, spleen, and lungs. Comparatively, Tra-AuNRs were mainly distributed in the tumor when intratumorally injected, with a tumor concentration of 6.42 μg/g after 24 h. The tumor growth inhibition rate reached 78.3% in the intratumoral injection group, with significantly higher BAX, BAD, and CASPASE-3 expression than that in the intravenous injection group. Conclusion: The findings suggest that Tra-AuNRs can be used for HER-2-positive gastric cancer treatment. Intratumoral injection of Tra-AuNRs significantly increased the local tumor drug concentration and improved the molecular targeted antitumor growth effect in gastric cancer-bearing nude mice. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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