RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity.

Autor: Vidal-Quist JC; Entomología Aplicada a la Agricultura y la Salud, Departamento de Biotecnología Microbiana y de Plantas, Centro de Investigaciones Biológicas Margarita Salas (CIB), CSIC, Madrid, Spain., Declercq J; Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium., Vanhee S; Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium., Lambrecht BN; Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium., Gómez-Rial J; Laboratorio de Inmunogenética, Unidad de Inmunología, Complejo Hospitalario Universitario de Santiago (CHUS), Santiago de Compostela, Spain., Vidal C; Servicio de Alergología, Complejo Hospitalario Universitario de Santiago (CHUS), Santiago de Compostela, Spain., Aydogdu E; Center for Plant Systems Biology, VIB, Ghent, Belgium.; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium., Rombauts S; Center for Plant Systems Biology, VIB, Ghent, Belgium.; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium., Hernández-Crespo P; Entomología Aplicada a la Agricultura y la Salud, Departamento de Biotecnología Microbiana y de Plantas, Centro de Investigaciones Biológicas Margarita Salas (CIB), CSIC, Madrid, Spain.
Jazyk: angličtina
Zdroj: Insect molecular biology [Insect Mol Biol] 2023 Apr; Vol. 32 (2), pp. 173-186. Date of Electronic Publication: 2023 Jan 04.
DOI: 10.1111/imb.12822
Abstrakt: RNA viruses have recently been detected in association with house dust mites, including laboratory cultures, dust samples, and mite-derived pharmaceuticals used for allergy diagnosis. This study aimed to assess the incidence of viral infection on Dermatophagoides pteronyssinus physiology and on the allergenic performance of extracts derived from its culture. Transcriptional changes between genetically identical control and virus-infected mite colonies were analysed by RNAseq with the support of a new D. pteronyssinus high-quality annotated genome (56.8 Mb, 108 scaffolds, N50 = 2.73 Mb, 96.7% BUSCO-completeness). Extracts of cultures and bodies from both colonies were compared by inspecting major allergen accumulation by enzyme-linked immunosorbent assay (ELISA), allergen-related enzymatic activities by specific assays, airway inflammation in a mouse model of allergic asthma, and binding to allergic patient's sera IgE by ImmunoCAP. Viral infection induced a significant transcriptional response, including several immunity and stress-response genes, and affected the expression of seven allergens, putative isoallergens and allergen orthologs. Major allergens were unaffected except for Der p 23 that was upregulated, increasing ELISA titers up to 29% in infected-mite extracts. By contrast, serine protease allergens Der p 3, 6 and 9 were downregulated, being trypsin and chymotrypsin enzymatic activities reduced up to 21% in extracts. None of the parameters analysed in our mouse model, nor binding to human IgE were significantly different when comparing control and infected-mite extracts. Despite the described physiological impact of viral infection on the mites, no significant consequences for the allergenicity of derived extracts or their practical use in allergy diagnosis have been detected.
(© 2022 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.)
Databáze: MEDLINE
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