| Autor: |
Tanyanskiy DA; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia. dmitry.athero@gmail.com.; Department of Fundamental Problems of Medicine and Medical Technologies, St. Petersburg State University, St. Petersburg, 199034, Russia., Shavva VS; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia., Dizhe EB; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia., Oleinikova GN; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia., Lizunov AV; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia.; Department of Embryology, St. Petersburg State University, St. Petersburg, 199034, Russia., Nekrasova EV; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia., Mogilenko DA; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia., Larionova EE; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia., Orlov SV; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia.; Department of Embryology, St. Petersburg State University, St. Petersburg, 199034, Russia., Denisenko AD; Department of Biochemistry, Institute of Experimental Medicine, St. Petersburg, 197376, Russia.; Department of Fundamental Problems of Medicine and Medical Technologies, St. Petersburg State University, St. Petersburg, 199034, Russia. |
| Abstrakt: |
Adiponectin is an adipose tissue hormone, participating in energy metabolism and involved in atherogenesis. Previously, it was found that adiponectin increases expression of the APOA1 (apolipoprotein A-1) gene in hepatocytes, but the mechanisms of this effect remained unexplored. Our aim was to investigate the role of adiponectin receptors AdipoR1/R2, AMP-activated protein kinase (AMPK), nuclear peroxisome proliferator-activated receptor alpha (PPARα) and liver X receptors (LXRs) in mediating the action of adiponectin on hepatic APOA1 expression in human hepatoma HepG2 cells. The level of APOA1 expression was determined by RT-qPCR and ELISA. We showed that the siRNA-mediated knockdown of genes coding for AdipoR1, AdipoR2, AMPK, PPARα, and LXRα and β prevented adiponectin-induced APOA1 expression in HepG2 cells and demonstrated that interaction of PPARα and LXRs with the APOA1 gene hepatic enhancer is important for the adiponectin-dependent APOA1 transcription. The results of this study point out to the involvement of both types of adiponectin receptors, AMPK, PPARα, and LXRs in the adiponectin-dependent upregulation of the APOA1 expression. |
