Comparative transcriptomic profiling of peach and nectarine cultivars reveals cultivar-specific responses to chilled postharvest storage.

Autor:	Muto A; Department of Biology, Ecology and Earth Sciences, University of Calabria, Cosenza, Italy., Bruno L; Department of Biology, Ecology and Earth Sciences, University of Calabria, Cosenza, Italy., Madeo ML; Department of Biology, Ecology and Earth Sciences, University of Calabria, Cosenza, Italy., Ludlow R; School of Biosciences, Cardiff University, Cardiff, United Kingdom., Ferrari M; Department of Biology, Ecology and Earth Sciences, University of Calabria, Cosenza, Italy., Stimpson L; Department of Biology, Ecology and Earth Sciences, University of Calabria, Cosenza, Italy.; School of Biosciences, Cardiff University, Cardiff, United Kingdom., LoGiudice C; Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Bari, Italy.; Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, Consiglio Nazionale delle Ricerche, Bari, Italy., Picardi E; Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Bari, Italy.; Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, Consiglio Nazionale delle Ricerche, Bari, Italy., Ferrante A; Department of Agricultural and Environmental Science, Università degli Studi di Milano, Milan, Italy., Pasti L; Department of Environment and Prevention Sciences, University of Ferrara, Ferrara, Italy., Müller CT; School of Biosciences, Cardiff University, Cardiff, United Kingdom., Chiappetta AAC; Department of Biology, Ecology and Earth Sciences, University of Calabria, Cosenza, Italy., Rogers HJ; School of Biosciences, Cardiff University, Cardiff, United Kingdom., Bitonti MB; Department of Biology, Ecology and Earth Sciences, University of Calabria, Cosenza, Italy., Spadafora ND; Department of Biology, Ecology and Earth Sciences, University of Calabria, Cosenza, Italy.; Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, Ferrara, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2022 Nov 25; Vol. 13, pp. 1062194. Date of Electronic Publication: 2022 Nov 25 (Print Publication: 2022).
DOI:	10.3389/fpls.2022.1062194
Abstrakt:	Introduction: Peach ( Prunus persica (L.) Batsch,) and nectarine fruits (Prunus persica (L.) Batsch, var nectarine), are characterized by a rapid deterioration at room temperature. Therefore, cold storage is widely used to delay fruit post-harvest ripening and extend fruit commercial life. Physiological disorders, collectively known as chilling injury, can develop typically after 3 weeks of low-temperature storage and affect fruit quality. Methods: A comparative transcriptomic analysis was performed to identify regulatory pathways that develop before chilling injury symptoms are detectable using next generation sequencing on the fruits of two contrasting cultivars, one peach (Sagittaria) and one nectarine, (Big Top), over 14 days of postharvest cold storage. Results: There was a progressive increase in the number of differentially expressed genes between time points (DEGs) in both cultivars. More (1264) time point DEGs were identified in 'Big Top' compared to 'Sagittaria' (746 DEGs). Both cultivars showed a downregulation of pathways related to photosynthesis, and an upregulation of pathways related to amino sugars, nucleotide sugar metabolism and plant hormone signal transduction with ethylene pathways being most affected. Expression patterns of ethylene related genes (including biosynthesis, signaling and ERF transcription factors) correlated with genes involved in cell wall modification, membrane composition, pathogen and stress response, which are all involved later during storage in development of chilling injury. Discussion: Overall, the results show that common pathways are activated in the fruit of 'Big Top' nectarine and 'Sagittaria' peach in response to cold storage but include also differences that are cultivar-specific responses. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Muto, Bruno, Madeo, Ludlow, Ferrari, Stimpson, LoGiudice, Picardi, Ferrante, Pasti, Müller, Chiappetta, Rogers, Bitonti and Spadafora.)
Databáze:	MEDLINE
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