Extrusion 3D printing of minicaplets for evaluating in vitro & in vivo praziquantel delivery capability.

Autor: Bhatt U; Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER)-Guwahati, Changsari-781101, Assam, India; National Centre for Pharmacoengineering, NIPER-Guwahati, Changsari-781101, Assam, India., Jorvekar SB; Department of Pharmaceutical Analysis, NIPER-Guwahati, Changsari-781101, Assam, India., Suryanarayana Murty U; National Centre for Pharmacoengineering, NIPER-Guwahati, Changsari-781101, Assam, India; NIPER-Guwahati, Changsari-781101, Assam, India., Borkar RM; Department of Pharmaceutical Analysis, NIPER-Guwahati, Changsari-781101, Assam, India. Electronic address: roshanudps@gmail.com., Banerjee S; Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER)-Guwahati, Changsari-781101, Assam, India; National Centre for Pharmacoengineering, NIPER-Guwahati, Changsari-781101, Assam, India. Electronic address: banerjee.subham@yahoo.co.in.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2023 Jan 05; Vol. 630, pp. 122445. Date of Electronic Publication: 2022 Nov 26.
DOI: 10.1016/j.ijpharm.2022.122445
Abstrakt: This study aimed to explore extrusion three dimensional (3D) printing technology to develop praziquantel (PZQ)-loaded minicaplets and evaluate their in vitro and in vivo delivery capabilities. PZQ-loaded minicaplets were 3D printed using a fused deposition modelling (FDM) principle-based extrusion 3D printer and were further characterized by different in vitro physicochemical and sophisticated analytical techniques. In addition, the % PZQ entrapment and in vitro PZQ release performance were evaluated using chromatographic techniques. It was in vitro observed that PZQ was fully released in the gastric pH medium within the period of gastric emptying, that is, 120 min, from the PZQ-loaded 3D printed minicaplets. Furthermore, in vivo pharmacokinetic (PK) profiles of PZQ-loaded 3D printed minicaplets were systematically evaluated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The PK profile of the PZQ-loaded 3D printed minicaplets was established using different parameters such as C max , T max , AUC 0-t , AUC 0-∞ , and oral relative bioavailability (RBA). The C max value of pristine PZQ was found at 64.79 ± 13.99 ng/ml, while PZQ-loaded 3D printed minicaplets showed a C max of 263.16 ± 47.85 ng/ml. Finally, the PZQ-loaded 3D printed minicaplets showed 9.0-fold improved oral RBA compared with that of pristine PZQ (1.0-fold). Together, these observations potentiate the desired in vitro and improved in vivo delivery capabilities of PZQ from the PZQ-loaded 3D printed minicaplets.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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