Cleavage kinetics is a better indicator of embryonic developmental competency than brilliant cresyl blue staining of oocytes.

Autor: Nix J; School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States., Marrella MA; School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States., Oliver MA; School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States., Rhoads M; School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States., Ealy AD; School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States., Biase FH; School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States. Electronic address: fbiase@vt.edu.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2023 Jan; Vol. 248, pp. 107174. Date of Electronic Publication: 2022 Dec 07.
DOI: 10.1016/j.anireprosci.2022.107174
Abstrakt: In vitro production of embryos (IVP) is a valuable technology to produce embryos of high genetic value. Despite advances in IVP, the efficiency of culture systems remains low. One method to increase IVP success is the early selection of oocytes or embryos that may have greater developmental potential. Here, we investigated two methods of selection, namely BCB staining and cleavage kinetics, both individually and in conjunction, for improved developmental outcomes in vitro. We hypothesized that a synergistic use of both BCB staining and cleavage kinetics would result in identification of embryos of greater developmental potential. The selection of oocytes by BCB staining does select for those oocytes with higher developmental potential, as noted by a greater blastocyst development between BCB positive (32.6%) and BCB negative (22.0%) on day 8 post-fertilization. However, the utilization of BCB staining and cleavage kinetics in tandem resulted in a complete masking of the effect observed when using BCB alone. We obtained the highest proportion of blastocyst development per selection group using cleavage kinetics alone, in which 53.1% of embryos grouped as Fast produced a blastocyst, which was significantly different from the three other groups (Fast+, Slow, not cleaved). We observed, however, that the separation of embryos by cleavage kinetics did not predict their survival to cryopreservation. In conclusion, in standard culture systems, cleavage kinetics is an effective method for the selection of embryos with increased developmental potential to develop blastocysts, however, it may not be effective to select healthy embryos for transfer following cryopreservation.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: