Quantitative Assessment of Serine-8 Phosphorylated β-Amyloid Using MALDI-TOF Mass Spectrometry.

Autor: Kuzin AA; Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia., Stupnikova GS; Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia., Strelnikova PA; Emanuel Institute of Biochemical Physics, Russian Academy of Science, 119334 Moscow, Russia.; Skolkovo Institute of Science and Technology, 121205 Moscow, Russia., Danichkina KV; Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia., Indeykina MI; Emanuel Institute of Biochemical Physics, Russian Academy of Science, 119334 Moscow, Russia.; Engelhardt Institute of Molecular Biology, 119991 Moscow, Russia., Pekov SI; Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia.; Skolkovo Institute of Science and Technology, 121205 Moscow, Russia.; Engelhardt Institute of Molecular Biology, 119991 Moscow, Russia.; Siberian State Medical University, 634050 Tomsk, Russia., Popov IA; Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia.; Siberian State Medical University, 634050 Tomsk, Russia.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2022 Dec 01; Vol. 27 (23). Date of Electronic Publication: 2022 Dec 01.
DOI: 10.3390/molecules27238406
Abstrakt: The study of the molecular mechanisms of the pathogenesis of Alzheimer's disease (AD) is extremely important for identifying potential therapeutic targets as well as early markers. In this regard, the study of the role of post-translational modifications (PTMs) of β-amyloid (Aβ) peptides is of particular relevance. Serine-8 phosphorylated forms (pSer8-Aβ) have been shown to have an increased aggregation capacity and may reflect the severity of amyloidosis. Here, an approach for quantitative assessment of pSer8-Aβ based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is proposed. The relative fraction of pSer8-Aβ was estimated in the total Aβ-pool with a detection limit of 1 fmol for pSer8-Aβ (1-16) and an accuracy of 2% for measurements in the reflectron mode. The sensitivity of the developed method is suitable for determining the proportion of phosphorylated peptides in biological samples.
Databáze: MEDLINE
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