Excess BAFF Alters NR4As Expression Levels and Breg Function of Human Precursor-like Marginal Zone B-Cells in the Context of HIV-1 Infection.

Autor: Doyon-Laliberté K; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC H2X 0A9, Canada.; Département de Microbiologie, Infectiologie et Immunologie de l'Université de Montréal, Montréal, QC H3T 1J4, Canada., Aranguren M; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC H2X 0A9, Canada.; Département de Microbiologie, Infectiologie et Immunologie de l'Université de Montréal, Montréal, QC H3T 1J4, Canada., Byrns M; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC H2X 0A9, Canada.; Département de Microbiologie, Infectiologie et Immunologie de l'Université de Montréal, Montréal, QC H3T 1J4, Canada., Chagnon-Choquet J; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC H2X 0A9, Canada.; Département de Microbiologie, Infectiologie et Immunologie de l'Université de Montréal, Montréal, QC H3T 1J4, Canada., Paniconi M; Service d'Aide à la Formation Interdisciplinaire et à la Réussite Étudiante (SAFIRE), Faculté des Arts et Sciences de l'Université de Montréal, Montréal, QC H3T 1N8, Canada., Routy JP; Department of Medicine, McGill University Health Centre, McGill University, Montréal, QC H4A 3J1, Canada., Tremblay C; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC H2X 0A9, Canada.; Département de Microbiologie, Infectiologie et Immunologie de l'Université de Montréal, Montréal, QC H3T 1J4, Canada., Quintal MC; Centre Hospitalier Ste-Justine de l'Université de Montréal, Montréal, QC H3T 1C5, Canada., Brassard N; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC H2X 0A9, Canada.; Département de Microbiologie, Infectiologie et Immunologie de l'Université de Montréal, Montréal, QC H3T 1J4, Canada., Kaufmann DE; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC H2X 0A9, Canada.; Département de Médecine de l'Université de Montréal, Montréal, QC H3T 1J4, Canada., Poudrier J; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC H2X 0A9, Canada.; Département de Microbiologie, Infectiologie et Immunologie de l'Université de Montréal, Montréal, QC H3T 1J4, Canada., Roger M; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC H2X 0A9, Canada.; Département de Microbiologie, Infectiologie et Immunologie de l'Université de Montréal, Montréal, QC H3T 1J4, Canada.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Dec 01; Vol. 23 (23). Date of Electronic Publication: 2022 Dec 01.
DOI: 10.3390/ijms232315142
Abstrakt: We have reported excess B-cell activating factor (BAFF) in the blood of HIV-infected progressors, which was concomitant with increased frequencies of precursor-like marginal zone (MZp) B-cells, early on and despite antiretroviral therapy (ART). In controls, MZp possess a strong B-cell regulatory (Breg) potential. They highly express IL-10, the orphan nuclear receptors (NR)4A1, NR4A2 and NR4A3, as well as the ectonucleotidases CD39 and CD73, all of which are associated with the regulation of inflammation. Furthermore, we have shown MZp regulatory function to involve CD83 signaling. To address the impact of HIV infection and excessive BAFF on MZp Breg capacities, we have performed transcriptomic analyses by RNA-seq of sorted MZp B-cells from the blood of HIV-infected progressors. The Breg profile and function of blood MZp B-cells from HIV-infected progressors were assessed by flow-cytometry and light microscopy high-content screening (HCS) analyses, respectively. We report significant downregulation of NR4A1, NR4A2, NR4A3 and CD83 gene transcripts in blood MZp B-cells from HIV-infected progressors when compared to controls. NR4A1, NR4A3 and CD83 protein expression levels and Breg function were also downregulated in blood MZp B-cells from HIV-infected progressors and not restored by ART. Moreover, we observe decreased expression levels of NR4A1, NR4A3, CD83 and IL-10 by blood and tonsillar MZp B-cells from controls following culture with excess BAFF, which significantly diminished their regulatory function. These findings, made on a limited number of individuals, suggest that excess BAFF contributes to the alteration of the Breg potential of MZp B-cells during HIV infection and possibly in other situations where BAFF is found in excess.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Databáze: MEDLINE
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