| Autor: |
Vasyutkina EA; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia., Yugay YA; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia., Grigorchuk VP; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia., Grishchenko OV; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia., Sorokina MR; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia., Yaroshenko YL; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia., Kudinova OD; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia., Stepochkina VD; Advanced Engineering School, Institute of Biotechnology, Bioengineering and Food Systems, Far Eastern Federal University, 10 Ajax Bay, Russky Island, Vladivostok 690922, Russia., Bulgakov VP; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia., Shkryl YN; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia. |
| Abstrakt: |
Ipomoea batatas is a vital root crop and a source of caffeoylquinic acid derivatives (CQAs) with potential health-promoting benefits. As a naturally transgenic plant, I. batatas contains cellular T-DNA (cT-DNA) sequence homologs of the Agrobacterium rhizogenes open reading frame (ORF)14, ORF17n, rooting locus (Rol)B/RolC, ORF13, and ORF18/ORF17n of unknown function. This study aimed to evaluate the effect of abiotic stresses (temperature, ultraviolet, and light) and chemical elicitors (methyl jasmonate, salicylic acid, and sodium nitroprusside) on the biosynthesis of CQAs and cT-DNA gene expression in I. batatas cell culture as a model system. Among all the applied treatments, ultraviolet irradiation, methyl jasmonate, and salicylic acid caused the maximal accumulation of secondary compounds. We also discovered that I. batatas cT-DNA genes were not expressed in cell culture, and the studied conditions weakly affected their transcriptional levels. However, the Ib-rolB/C gene expressed under the strong 35S CaMV promoter increased the CQAs content by 1.5-1.9-fold. Overall, our results show that cT-DNA-encoded transgenes are not involved in stress- and chemical elicitor-induced CQAs accumulation in cell cultures of I. batatas . Nevertheless, overaccumulation of RolB/RolC transcripts potentiates the secondary metabolism of sweet potatoes through a currently unknown mechanism. Our study provides new insights into the molecular mechanisms linked with CQAs biosynthesis in cell culture of naturally transgenic food crops, i.e., sweet potato. |
