| Autor: |
Bustamante CC; Department of Veterinary Medicine and Surgery, School of Agricultural and Veterinary Sciences, UNESP-São Paulo State University, Jaboticabal 14884-900, SP, Brazil., de Paula VB; Department of Veterinary Medicine and Surgery, School of Agricultural and Veterinary Sciences, UNESP-São Paulo State University, Jaboticabal 14884-900, SP, Brazil., Rabelo IP; Department of Veterinary Medicine and Surgery, School of Agricultural and Veterinary Sciences, UNESP-São Paulo State University, Jaboticabal 14884-900, SP, Brazil., Fernandes CC; Department of Technology, Multiuse Sequencing Laboratory, School of Agricultural and Veterinary Sciences, UNESP-São Paulo State University, Jaboticabal 14884-900, SP, Brazil., Kishi LT; Department of Technology, Multiuse Sequencing Laboratory, School of Agricultural and Veterinary Sciences, UNESP-São Paulo State University, Jaboticabal 14884-900, SP, Brazil., Canola PA; Department of Veterinary Medicine and Surgery, School of Agricultural and Veterinary Sciences, UNESP-São Paulo State University, Jaboticabal 14884-900, SP, Brazil., Lemos EGM; Department of Technology, Biochemistry of Microorganisms and Plants Laboratory, School of Agricultural and Veterinary Sciences, UNESP-São Paulo State University, Jaboticabal 14884-900, SP, Brazil., Valadão CAA; Department of Veterinary Medicine and Surgery, School of Agricultural and Veterinary Sciences, UNESP-São Paulo State University, Jaboticabal 14884-900, SP, Brazil. |
| Abstrakt: |
Starch overload in horses causes gastrointestinal and metabolic disorders that are associated with microbiota changes. Therefore, we identified the fecal microbiota and hypothesized that intracecal injection of alkaline solution (buffer; Mg(OH)2 + Al(OH)3) could stabilize these microbiota and clinical changes in horses submitted to corn starch overload. Ten crossbred horses (females and geldings) were allocated to group I (water−saline and starch−buffer treatments) and group II (water−buffer and starch−saline treatments). Clinical signs, gross analysis of the feces, and fecal microbiota were evaluated through 72 h (T0; T8; T12; T24; T48; T72). Corn starch or water were administrated by nasogastric tube at T0, and the buffer injected into the cecum at T8 in starch−buffer and water−buffer treatments. Starch overload reduced the richness (p < 0.001) and diversity (p = 0.001) of the fecal microbiota. However, the starch−buffer treatment showed greater increase in amylolytic bacteria (Bifidobacterium 0.0% to 5.6%; Lactobacillus 0.1% to 7.4%; p < 0.05) and decrease in fibrolytic bacteria (Lachnospiraceae 10.2% to 5.0%; Ruminococcaceae 11.7% to 4.2%; p < 0.05) than starch−saline treatment. Additionally, animals that received starch−buffer treatment showed more signs of abdominal discomfort and lameness associated with dysbiosis (amylolytic r > 0.5; fribolytic r < 0.1; p < 0.05), showing that cecal infusion of buffer did not prevent, but intensified intestinal disturbances and the risk of laminitis. |
