Skeletal muscle insulin resistance and adipose tissue hypertrophy persist beyond the reshaping of gut microbiota in young rats fed a fructose-rich diet.

Autor: Mazzoli A; Department of Biology, University of Naples Federico II, Naples, Italy., Porzio AD; Department of Biology, University of Naples Federico II, Naples, Italy., Gatto C; Department of Biology, University of Naples Federico II, Naples, Italy., Crescenzo R; Department of Biology, University of Naples Federico II, Naples, Italy; Task Force on Microbiome Studies, University of Naples Federico II, Naples, Italy., Nazzaro M; Department of Biology, University of Naples Federico II, Naples, Italy., Spagnuolo MS; Department of Bio-Agrofood Science, Institute for the Animal Production System in Mediterranean Environment, National Research Council Naples (CNR-ISPAAM), Naples, Italy., Baccigalupi L; Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy; Task Force on Microbiome Studies, University of Naples Federico II, Naples, Italy., Ricca E; Department of Biology, University of Naples Federico II, Naples, Italy; Task Force on Microbiome Studies, University of Naples Federico II, Naples, Italy., Amoresano A; Department of Chemical Sciences, University of Naples Federico II, Naples, Italy., Fontanarosa C; Department of Chemical Sciences, University of Naples Federico II, Naples, Italy., Bernacchioni C; Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Florence, Italy., Donati C; Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Florence, Italy., Iossa S; Department of Biology, University of Naples Federico II, Naples, Italy; Task Force on Microbiome Studies, University of Naples Federico II, Naples, Italy. Electronic address: susiossa@unina.it., Cigliano L; Department of Biology, University of Naples Federico II, Naples, Italy; Task Force on Microbiome Studies, University of Naples Federico II, Naples, Italy.
Jazyk: angličtina
Zdroj: The Journal of nutritional biochemistry [J Nutr Biochem] 2023 Mar; Vol. 113, pp. 109247. Date of Electronic Publication: 2022 Dec 07.
DOI: 10.1016/j.jnutbio.2022.109247
Abstrakt: To investigate whether short term fructose-rich diet induces changes in the gut microbiota as well as in skeletal muscle and adipose tissue physiology and verify whether they persist even after fructose withdrawal, young rats of 30 d of age were fed for 3 weeks a fructose-rich or control diet. At the end of the 3-weeks period, half of the rats from each group were maintained for further 3 weeks on a control diet. Metagenomic analysis of gut microbiota and short chain fatty acids levels (faeces and plasma) were investigated. Insulin response was evaluated at the whole-body level and both in skeletal muscle and epididymal adipose tissue, together with skeletal muscle mitochondrial function, oxidative stress, and lipid composition. In parallel, morphology and physiological status of epididymal adipose tissue was also evaluated. Reshaping of gut microbiota and increased content of short chain fatty acids was elicited by the fructose diet and abolished by switching back to control diet. On the other hand, most metabolic changes elicited by fructose-rich diet in skeletal muscle and epididymal adipose tissue persisted after switching to control diet. Increased dietary fructose intake even on a short-time basis elicits persistent changes in the physiology of metabolically relevant tissues, such as adipose tissue and skeletal muscle, through mechanisms that go well beyond the reshaping of gut microbiota. This picture delineates a harmful situation, in particular for the young populations, posed at risk of metabolic modifications that may persist in their adulthood.
Competing Interests: Declaration of Competing Interests The authors declare no conflict of interest.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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