Inside and outside of virus-like particles HBc and HBc/4M2e: A comprehensive study of the structure.

Autor: Egorov VV; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, Russian Federation; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, Prof. Popov St. 15/17, St. Petersburg, Russian Federation; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl. 1, 123182 Moscow, Russian Federation; Institute of Experimental Medicine, Academika Pavlova, 12, 197376 St. Petersburg, Russian Federation. Electronic address: sondyn@yandex.ru., Shvetsov AV; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, Russian Federation; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl. 1, 123182 Moscow, Russian Federation; Peter the Great St.Petersburg Polytechnic University, Politehnicheskaya 29, St. Petersburg, Russian Federation., Pichkur EB; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl. 1, 123182 Moscow, Russian Federation., Shaldzhyan AA; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, Prof. Popov St. 15/17, St. Petersburg, Russian Federation., Zabrodskaya YA; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, Prof. Popov St. 15/17, St. Petersburg, Russian Federation; Peter the Great St.Petersburg Polytechnic University, Politehnicheskaya 29, St. Petersburg, Russian Federation., Vinogradova DS; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, Russian Federation., Nekrasov PA; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, Prof. Popov St. 15/17, St. Petersburg, Russian Federation., Gorshkov AN; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, Prof. Popov St. 15/17, St. Petersburg, Russian Federation., Garmay YP; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, Russian Federation., Kovaleva AA; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, Prof. Popov St. 15/17, St. Petersburg, Russian Federation., Stepanova LA; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, Prof. Popov St. 15/17, St. Petersburg, Russian Federation., Tsybalova LM; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, Prof. Popov St. 15/17, St. Petersburg, Russian Federation., Shtam TA; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, Russian Federation; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl. 1, 123182 Moscow, Russian Federation., Myasnikov AG; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, Russian Federation; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl. 1, 123182 Moscow, Russian Federation., Konevega AL; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, Russian Federation; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl. 1, 123182 Moscow, Russian Federation; Peter the Great St.Petersburg Polytechnic University, Politehnicheskaya 29, St. Petersburg, Russian Federation.
Jazyk: angličtina
Zdroj: Biophysical chemistry [Biophys Chem] 2023 Feb; Vol. 293, pp. 106943. Date of Electronic Publication: 2022 Dec 05.
DOI: 10.1016/j.bpc.2022.106943
Abstrakt: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (HBc/4M2e) form virus-like particles whose structure was studied using a combination of molecular modeling and cryo-electron microscopy (cryo-EM). It was also shown that self-assembling of the particles occurs inside bacterial cells, but despite the big inner volume of the core shell particle, purified HBc/4M2e contain an insignificant amount of bacterial proteins. It was shown that a fragment of the M2e corresponding to 4M2e insertion is prone to formation of amyloid-like fibrils. However, as the part of the immunodominant loop, M2e insertion does not show a tendency to intermolecular interaction. A full-atomic HBc-4M2e model with the resolution of about 3 Å (3.13 Å for particles of Т = 4 symmetry, 3.7 Å for particles of Т = 3 symmetry) was obtained by molecular modeling methods based on cryo-EM data.
Competing Interests: Declaration of Competing Interest The authors have declared that no competing interests exist.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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