Prevalence of the Direct Antiglobulin Test and Its Clinical Impact on Multiply Transfused Thalassemia Patients: A Prospective Study Conducted at a Tertiary Care Center in Northern India.

Autor:	Kumar Yadav B; Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute Medical Sciences, Lucknow, India., Chaudhary RK; Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute Medical Sciences, Lucknow, India., Shrivastava H; Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute Medical Sciences, Lucknow, India., Elhence P; Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute Medical Sciences, Lucknow, India.
Jazyk:	angličtina
Zdroj:	Laboratory medicine [Lab Med] 2023 Jul 05; Vol. 54 (4), pp. 406-410.
DOI:	10.1093/labmed/lmac140
Abstrakt:	Objective: This study was conducted to estimate prevalence of direct antiglobulin test (DAT) positivity and its impact on transfusion support in patients with thalassemia. Methods: The DAT testing was performed for patients with β-thalassemia who received transfusion from November 2021 to March 2022. Elution was done for DAT-positive samples. Results: Of 180 patients, 21 (11.6%) were DAT positive. Immunoglobulin G (IgG) was present in 4 (19%) and IgG+C3d was present in 8 (38%). Only complement was present in 9 (42.8%) patients. The IgG-reactive DATs were associated with pan-reactive eluate. Patients who were DAT-positive had significantly higher levels of serum bilirubin, ferritin, and IgG than those who were DAT-negative. Conclusion: Autoantibody formation in multiply transfused thalassemia patients is common and merits equal attention as alloimmunization. It is particularly important as DAT-positive red blood cells may undergo clinically significant hemolysis, which may increase the transfusion requirements with associated sequalae such as increased serum ferritin and splenomegaly. (© The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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