| Autor: |
Krivorutchenko YL; Medical Academy named after S.I. Georgievsky, Federal State Autonomous Educational Institution of Higher Education «V. I. Vernadsky Crimean Federal University»., Nosik DN; D.I. Ivanovsky Institute of Virology, «National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»., Maligina VY; Medical Academy named after S.I. Georgievsky, Federal State Autonomous Educational Institution of Higher Education «V. I. Vernadsky Crimean Federal University»., Lobach OA; D.I. Ivanovsky Institute of Virology, «National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»., Andronovskaja IB; Medical Academy named after S.I. Georgievsky, Federal State Autonomous Educational Institution of Higher Education «V. I. Vernadsky Crimean Federal University»., Kirsanova MA; Medical Academy named after S.I. Georgievsky, Federal State Autonomous Educational Institution of Higher Education «V. I. Vernadsky Crimean Federal University»., Grishkovets VI; Medical Academy named after S.I. Georgievsky, Federal State Autonomous Educational Institution of Higher Education «V. I. Vernadsky Crimean Federal University». |
| Jazyk: |
angličtina |
| Zdroj: |
Voprosy virusologii [Vopr Virusol] 2018 Jun 20; Vol. 63 (3), pp. 123-129. Date of Electronic Publication: 2018 Jun 20. |
| DOI: |
10.18821/0507-4088-2018-63-3-123-129 |
| Abstrakt: |
Triterpene saponin Taurosid Sx1 purified from leaves of the plant Crimean Ivy Hedera taurica Carr. (Araliaceae) was evaluated for its cytotoxic activity against lymphoblastoid cell lines MT-4, Jurkat-tat, U937, and human peripheral blood monocytes. The ability of saponin to influence HIV-1 replication was studied as well. In addition, the ability of Taurosid Sx1 to increase survival of mice infected with influenza virus А/WSN/1/33(H1N1) and its capacity for strengthening the immune responses in mice immunized with the influenza vaccine Grippol® have been studied. Taurosid Sx1 has been shown to inhibit MT-4 cell line at 25 μg ml-1 concentration, IC50 33,3 μmol l-1 (MTT assay). The saponin concentration of 5 μg ml-1 was non-toxic for all the cell lines studied and demonstrated a moderate inhibitory effect on HIV p24 production in Jurkat-tat cells. In the lower concentrations Taurosid Sx1 did not stimulate HIV p24 production. It was shown that oral administration of 200 μg Taurosid Sx1 to the influenza virus infected mice caused 1.5-fold increase in their survival. Taurosid Sx1 given orally amplified immunopotentiating ability of an intramusculary administered subunit influenza vaccine. Antibody production was significantly higher in animals fed Taurosid Sx1 after primary or secondary immunizatuion. In mice given 2 doses of vaccine, from 1 to 3 weeks apart, feeding 200 μg saponin resulted in 2 to 10-fold enhancement in production of anti-H1, anti-H3, and anti-inluenza type B hemagglutinin antibodies. Thus, Taurosid Sx1 can be considered as low-toxic promising immunopotentiating agent uncapable of enhancing HIV-1 replication. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
