Low-density lipoprotein cholesterol and non-high-density lipoprotein cholesterol measurement in Familial Dysbetalipoproteinemia.

Autor: Heidemann BE; Department of Vascular Medicine, University Medical Center Utrecht, Utrecht University, The Netherlands., Koopal C; Department of Vascular Medicine, University Medical Center Utrecht, Utrecht University, The Netherlands., Roeters van Lennep JE; Department of Internal Medicine, Erasmus Medical Center, Rotterdam, The Netherlands., Stroes ES; Department of Vascular Medicine, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands., Riksen NP; Department of Internal Medicine and Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands., Mulder MT; Department of Internal Medicine, Division of Pharmacology, Vascular and Metabolic Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands., van Vark-van der Zee LC; Department of Internal Medicine, Division of Pharmacology, Vascular and Metabolic Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands., Blackhurst DM; Division of Chemical Pathology, Faculty of Health Sciences, University of Cape Town, South Africa, Cape Town, South Africa., Visseren FLJ; Department of Vascular Medicine, University Medical Center Utrecht, Utrecht University, The Netherlands. Electronic address: f.l.j.visseren@umcutrecht.nl., Marais AD; Division of Chemical Pathology, Faculty of Health Sciences, University of Cape Town, South Africa, Cape Town, South Africa.
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2023 Jan 15; Vol. 539, pp. 114-121. Date of Electronic Publication: 2022 Dec 06.
DOI: 10.1016/j.cca.2022.11.035
Abstrakt: Aim: To compare LDL-C concentrations using the Friedewald formula, the Martin-Hopkins formula, a direct assay and polyacrylamide gradient gel electrophoresis (PGGE) to the reference standard density gradient ultracentrifugation in patients with Familial Dysbetalipoproteinemia (FD) patients. We also compared non-HDL-cholesterol concentrations by two methods.
Methods: For this study data from 28 patients with genetically confirmed FD from the placebo arm of the EVOLVE-FD trial were used. Four different methods for determining LDL-C were compared with ultracentrifugation. Non-HDL-C was measured with standard assays and compared to ultracentrifugation. Correlation coefficients and Bland-Altman plots were used to compare the methods.
Results: Mean age of the 28 FD patients was 62 ± 9 years, 43 % were female and 93 % had an ɛ2ɛ2 genotype. LDL-C determined by Friedewald (R 2 = 0.62, p <0.01), Martin-Hopkins (R 2 = 0.50, p = 0.01) and the direct assay (R 2 = 0.41, p = 0.03) correlated with density gradient ultracentrifugation. However, Bland-Altman plots showed considerable over- or underestimation by the four methods compared to ultracentrifugation. Non-HDL-C showed good correlation and agreement.
Conclusion: In patients with FD, all four methods investigated over- or underestimated LDL-C concentrations compared with ultracentrifugation. In contrast, standard non-HDL-C assays performed well, emphasizing the use of non-HDL-C in patients with FD.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: BEH declares no conflicts of interest. CK declares no conflicts of interest. JRvL has received a research grant by Amryt. Ad-board speaker fees have been paid to institution of ES by: Amgen, Sanofi, Esperion, Novo-Nordisk, Akcea/Ionis, Regeneron. NR declares no conflict of interest. MM declares no conflicts of interest LvZ declares no conflicts of interest. DMB declares no conflict of interest. ADM declares no conflict of interest. FV declares no conflict of interest.
(Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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