Analyzing the Impact of Ramalina digitellata, R. fastigiata, R. fraxinea, and R. polymorpha's Usnic Acid Concentration on Antioxidant, DNA-Protective, Antimicrobial, and Cytotoxic Properties.

Autor: Kiliç Yayla S; Institute of Science, Yozgat Bozok University, Yozgat, Turkey., Kocakaya Z; Department of Plant and Animal Production, Safiye Çıkrıkçıoğlu Vocational School, Kayseri University, Kayseri, Turkey., Karatoprak GŞ; Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey., İlgün S; Department of Pharmaceutical Botany, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey., Ceylan A; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2023 Jan; Vol. 20 (1), pp. e202200816. Date of Electronic Publication: 2023 Jan 05.
DOI: 10.1002/cbdv.202200816
Abstrakt: The present study is focused on the antimicrobial, antioxidant, cytotoxic, and DNA protective effects of methanol extract obtained from R. digitellata, R. fastigiata, R. fraxinea, and R. polymorpha species that are distributed in Turkey. The highest total phenol content was determined in R. digitellata (144.6 mgGAE/g extract ), and the highest total amount of flavonoids was found in R. fastigiata (20.40 mgGAE/g extract ). The content of usnic acid was determined by High-Performance Liquid Chromatography (HPLC) and the highest amount was found in R. digitellata. DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS [2,2'-azinobis(3-ethylbenzathiazoline-6-sulfonic acid)] radical scavenging methods were used for antioxidant activity. R. fraxinea showed the highest DPPH⋅ and ABTS + ⋅ scavenging activity. In addition, the DNA protective effect was investigated using pBR322 plasmid DNA, and; all studied species were found to have DNA protective effects. The antibacterial activity was investigated using the disc diffusion method, and the R. digitellata methanol extract showed the best results with a 12.35 mm zone on Proteus mirabilis. On the human lung cancer (A549) and breast cancer (MDA-MB-231) cell lines, cytotoxic activity was assessed using an MTT assay. All lichen extracts were found to have a significant cytotoxic effect on both cancer cell lines at 1000 μg/mL concentration. These results suggest that Ramalina species may be potential candidates for developing new phytopharmaceuticals and functional components.
(© 2022 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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