Lymphatic Vascular Density, the Expression of Podoplanin and Tumor Budding in Oral Squamous Cell Carcinoma.

Autor: de Assis EM; Oral Pathology Section and Graduate Program in Dentistry, Pontifícia Universidade Católica de Minas Gerais (PUC Minas), Belo Horizonte, MG, Brazil.; Faculdade Pitágoras de Ipatinga, Ipatinga, MG, Brazil., Rodrigues M; Oral Pathology Section and Graduate Program in Dentistry, Pontifícia Universidade Católica de Minas Gerais (PUC Minas), Belo Horizonte, MG, Brazil., Vieira JC; Oral Pathology Section and Graduate Program in Dentistry, Pontifícia Universidade Católica de Minas Gerais (PUC Minas), Belo Horizonte, MG, Brazil., Pascoaloti MIM; Oral Pathology Section and Graduate Program in Dentistry, Pontifícia Universidade Católica de Minas Gerais (PUC Minas), Belo Horizonte, MG, Brazil., Junior HM; Oral Pathology Section and Graduate Program in Dentistry, Pontifícia Universidade Católica de Minas Gerais (PUC Minas), Belo Horizonte, MG, Brazil.; Centro Universitário de Patos de Minas (UNIPAM), Patos de Minas, MG, Brazil., Souto GR; Oral Pathology Section and Graduate Program in Dentistry, Pontifícia Universidade Católica de Minas Gerais (PUC Minas), Belo Horizonte, MG, Brazil.; Departamento de Odontologia - Pontifícia Universidade Católica de Minas Gerais, Avenida Dom José Gaspar 500, Prédio 46, Sala 101, Belo Horizonte, Minas Gerais, CEP: 30535-901, Brasil., Souza PEA; Oral Pathology Section and Graduate Program in Dentistry, Pontifícia Universidade Católica de Minas Gerais (PUC Minas), Belo Horizonte, MG, Brazil.; Departamento de Odontologia - Pontifícia Universidade Católica de Minas Gerais, Avenida Dom José Gaspar 500, Prédio 46, Sala 101, Belo Horizonte, Minas Gerais, CEP: 30535-901, Brasil., Horta MCR; Oral Pathology Section and Graduate Program in Dentistry, Pontifícia Universidade Católica de Minas Gerais (PUC Minas), Belo Horizonte, MG, Brazil. martinhohorta@pucminas.br.; Departamento de Odontologia - Pontifícia Universidade Católica de Minas Gerais, Avenida Dom José Gaspar 500, Prédio 46, Sala 101, Belo Horizonte, Minas Gerais, CEP: 30535-901, Brasil. martinhohorta@pucminas.br.
Jazyk: angličtina
Zdroj: Head and neck pathology [Head Neck Pathol] 2023 Jun; Vol. 17 (2), pp. 371-382. Date of Electronic Publication: 2022 Dec 08.
DOI: 10.1007/s12105-022-01511-z
Abstrakt: Background: Notwithstanding recent advances in oral squamous cell carcinoma (OSCC) management, its mortality rate is still high. It is imperative to investigate new parameters that are complementary to clinical staging for OSCC to provide better prognostic insight. The presence of isolated neoplastic cells or small clusters of up to four cells at the tumor's invasive front, called tumor budding, is a morphological marker of OSCC with prognostic value. Increased lymphatic vascular density (LVD) and a high expression of podoplanin in neoplastic cells have also been associated with worse prognosis in OSCC. To investigate these markers in OSCC, we evaluated differences in LVD and the expression of podoplanin in neoplastic cells between tumors with high-intensity tumor budding versus low-intensity or no tumor budding. In the samples of high-intensity budding, differences in those parameters between the​​ budding area and the area outside the budding were also evaluated. Furthermore, the study assessed differences in LVD and in the expression of podoplanin in neoplastic cells concerning OSCC clinicopathological characteristics.
Methods: To those ends, we subjected 150 samples of OSCC to immunohistochemistry to evaluate the intensity of tumor budding (via multi-cytokeratin immunostaining). Moreover, the 150 samples of OSCC and 15 specimens of normal oral mucosa (used as a control) were employed to assess LVD and the expression of podoplanin (in neoplastic cells of OSCC and in the lining epithelium of normal oral mucosa), both via podoplanin immunostaining. Data were processed into descriptive and analytical statistics.
Results: No differences were observed neither in the LVD nor in the expression of podoplanin in neoplastic cells concerning sex, age, tobacco smoking, tumor location and tumor size. The LVD was greater in OSCC and in tumors with high-intensity budding than in normal mucosa but did not differ between normal mucosa and tumors with low-intensity or no tumor budding. The data analyses also revealed that LVD was greater in tumors with high-intensity tumor budding than in tumors with low-intensity or no budding and showed no difference in LVD between the budding area and the area outside the budding. When compared to the lining epithelium of the normal mucosa, the expression of podoplanin was greater in neoplastic cells of OSCC, tumors with high-intensity budding and tumors with low-intensity or no tumor budding. The expression of podoplanin in neoplastic cells was also greater in tumors with high-intensity budding and, within those tumors, greater in the budding area than in the area outside de budding.
Conclusion: Those findings support the hypothesis that tumor budding is a biological phenomenon associated with the progression and biological behavior of OSCC.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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