High-throughput identification of novel heat tolerance genes via genome-wide pooled mutant screens in the model green alga Chlamydomonas reinhardtii.

Autor: Mattoon EM; Donald Danforth Plant Science Center, St. Louis, Missouri, USA.; Plant and Microbial Biosciences Program, Division of Biology and Biomedical Sciences, Washington University in Saint Louis, St. Louis, Missouri, USA., McHargue WE; Donald Danforth Plant Science Center, St. Louis, Missouri, USA., Bailey CE; Donald Danforth Plant Science Center, St. Louis, Missouri, USA., Zhang N; Donald Danforth Plant Science Center, St. Louis, Missouri, USA., Chen C; Department of Electrical Engineering and Computer Science, University of Missouri, Columbia, Missouri, USA., Eckhardt J; Donald Danforth Plant Science Center, St. Louis, Missouri, USA., Daum CG; U.S. Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Zane M; U.S. Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Pennacchio C; U.S. Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Schmutz J; U.S. Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA.; HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA., O'Malley RC; U.S. Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA.; Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Cheng J; Department of Electrical Engineering and Computer Science, University of Missouri, Columbia, Missouri, USA., Zhang R; Donald Danforth Plant Science Center, St. Louis, Missouri, USA.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2023 Mar; Vol. 46 (3), pp. 865-888. Date of Electronic Publication: 2022 Dec 24.
DOI: 10.1111/pce.14507
Abstrakt: Different high temperatures adversely affect crop and algal yields with various responses in photosynthetic cells. The list of genes required for thermotolerance remains elusive. Additionally, it is unclear how carbon source availability affects heat responses in plants and algae. We utilized the insertional, indexed, genome-saturating mutant library of the unicellular, eukaryotic green alga Chlamydomonas reinhardtii to perform genome-wide, quantitative, pooled screens under moderate (35°C) or acute (40°C) high temperatures with or without organic carbon sources. We identified heat-sensitive mutants based on quantitative growth rates and identified putative heat tolerance genes (HTGs). By triangulating HTGs with heat-induced transcripts or proteins in wildtype cultures and MapMan functional annotations, we presented a high/medium-confidence list of 933 Chlamydomonas genes with putative roles in heat tolerance. Triangulated HTGs include those with known thermotolerance roles and novel genes with little or no functional annotation. About 50% of these high-confidence HTGs in Chlamydomonas have orthologs in green lineage organisms, including crop species. Arabidopsis thaliana mutants deficient in the ortholog of a high-confidence Chlamydomonas HTG were also heat sensitive. This work expands our knowledge of heat responses in photosynthetic cells and provides engineering targets to improve thermotolerance in algae and crops.
(© 2022 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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