NUSAP1 and PCLAF ( KIA0101 ) Downregulation by Neoadjuvant Therapy is Associated with Better Therapeutic Outcomes and Survival in Breast Cancer.

Autor: Magallanes-Garza GI; Tecnologico de Monterrey, Escuela de Medicina y Ciencias de La Salud, Monterrey, Mexico.; Tecnologico de Monterrey, Servicio de Ginecologia y Obstetricia, Tec Salud, Monterrey, Mexico.; Tecnologico de Monterrey, Centro de Cancer de Mama, Hospital Zambrano Hellion, San Pedro Garza Garcia, Mexico., Santuario-Facio SK; Tecnologico de Monterrey, Escuela de Medicina y Ciencias de La Salud, Monterrey, Mexico., Lira-Albarrán S; Tecnologico de Monterrey, The Institute for Obesity Research, Monterrey, Mexico., Varela-Varela AF; Universidad Autonoma de Coahuila, Centro de Investigacion Biomedica, Facultad de Medicina, Unidad Torreon, Torreon, Mexico., Cardona-Huerta S; Tecnologico de Monterrey, Direccion Nacional de Investigacion Clinica TecSalud, Monterrey, Mexico.; Instituto Mexicano Del Seguro Social, Unidad Medica de Alta Especialidad No. 25, Monterrey, Mexico., Ruiz-Flores P; Universidad Autonoma de Coahuila, Centro de Investigacion Biomedica, Facultad de Medicina, Unidad Torreon, Torreon, Mexico., Haro-Santa-Cruz J; Universidad Autonoma de Coahuila, Centro de Investigacion Biomedica, Facultad de Medicina, Unidad Torreon, Torreon, Mexico., Perez-Paramo YX; Washington State University, Pharmaceutical Sciences Department, College of Pharmacy Spokane, WA, USA., Gomez-Macias GS; Tecnologico de Monterrey, Centro de Cancer de Mama, Hospital Zambrano Hellion, San Pedro Garza Garcia, Mexico.; Universidad Autonoma de Nuevo Leon, Facultad de Medicina, Monterrey, Mexico., Davila-Gonzalez D; Tecnologico de Monterrey, Escuela de Medicina y Ciencias de La Salud, Monterrey, Mexico.; Tecnologico de Monterrey, Direccion Nacional de Investigacion Clinica TecSalud, Monterrey, Mexico., Valero-Gomez J; Tecnologico de Monterrey, Escuela de Medicina y Ciencias de La Salud, Monterrey, Mexico., Borrego-Soto G; University of Texas at Austin, Department of Molecular Biosciences, Austin, TX, USA., Rojas-Martinez A; Tecnologico de Monterrey, Escuela de Medicina y Ciencias de La Salud, Monterrey, Mexico.; Tecnologico de Monterrey, The Institute for Obesity Research, Monterrey, Mexico.; Universidad Autonoma de Nuevo Leon, CIDICS, Monterrey, Mexico., Ortiz-Lopez R; Tecnologico de Monterrey, Escuela de Medicina y Ciencias de La Salud, Monterrey, Mexico.; Tecnologico de Monterrey, The Institute for Obesity Research, Monterrey, Mexico.; Universidad Autonoma de Nuevo Leon, CIDICS, Monterrey, Mexico.
Jazyk: angličtina
Zdroj: Journal of oncology [J Oncol] 2022 Nov 28; Vol. 2022, pp. 6001947. Date of Electronic Publication: 2022 Nov 28 (Print Publication: 2022).
DOI: 10.1155/2022/6001947
Abstrakt: Purpose: To evaluate whether changes in genomic expression that occur beginning with breast cancer (BC) diagnosis and through to tumor resection after neoadjuvant chemotherapy (NCT) reveal biomarkers that can help predict therapeutic response and survival.
Materials and Methods: We determined gene expression profiles based on microarrays in tumor samples from 39 BC patients who showed pathologic complete response (pCR) or therapeutic failure (non-pCR) after NCT (cyclophosphamide-doxorubicin/epirubicin). Based on unsupervised clustering of gene expression, together with functional enrichment analyses of differentially expressed genes, we selected NUSAP1 , PCLAF , MME , and DST . We evaluated the NCT response and the expression of these four genes in BC histologic subtypes. In addition, we study the presence of tumor-infiltrating lymphocytes. Finally, we analyze the correlation between NUSAP1 and PCLAF against disease-free survival (DFS) and overall survival (OS).
Results: A signature of 43 differentially expressed genes discriminated pCR from non-pCR patients (|fold change >2|, false discovery rate <0.05) only in biopsies taken after surgery. Patients achieving pCR showed downregulation of NUSAP1 and PCLAF in tumor tissues and increased DFS and OS, while overexpression of these genes correlated with poor therapeutic response and OS. These genes are involved in the regulation of mitotic division.
Conclusions: The downregulation of NUSAP1 and PCLAF after NCT is associated with the tumor response to chemotherapy and patient survival.
Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this paper.
(Copyright © 2022 Gerardo I. Magallanes-Garza et al.)
Databáze: MEDLINE
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