Decellularization of cartilage microparticles: Effects of temperature, supercritical carbon dioxide and ultrasound on biochemical, mechanical, and biological properties.

Autor: Sevastianov VI; Department for Biomedical Technologies and Tissue Engineering, The Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia.; The Institute of Biomedical Research and Technology, Moscow, Russia., Basok YB; Department for Biomedical Technologies and Tissue Engineering, The Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia., Grigoriev AM; Department for Biomedical Technologies and Tissue Engineering, The Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia., Nemets EA; Department for Biomedical Technologies and Tissue Engineering, The Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia., Kirillova AD; Department for Biomedical Technologies and Tissue Engineering, The Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia., Kirsanova LA; Department for Biomedical Technologies and Tissue Engineering, The Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia., Lazhko AE; Chemical Department, Lomonosov Moscow State University, Moscow, Russia., Subbot A; Laboratory of Fundamental Research in Ophtalmology, The Research Institute of Eye Diseases, Moscow, Russia., Kravchik MV; Laboratory of Fundamental Research in Ophtalmology, The Research Institute of Eye Diseases, Moscow, Russia., Khesuani YD; Laboratory for Biotechnological Research '3D Bioprinting Solutions', Moscow, Russia., Koudan EV; Center for Biomedical Engineering, National University of Science and Technology 'MISIS', Moscow, Russia., Gautier SV; Department for Biomedical Technologies and Tissue Engineering, The Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia.; The Department of Transplantology and Artificial Organs, Faculty of Medicine, The Sechenov University, Moscow, Russia.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2023 Apr; Vol. 111 (4), pp. 543-555. Date of Electronic Publication: 2022 Dec 07.
DOI: 10.1002/jbm.a.37474
Abstrakt: One of the approaches to restoring the structure of damaged cartilage tissue is an intra-articular injection of tissue-engineered medical products (TEMPs) consisting of biocompatible matrices loaded with cells. The most interesting are the absorbable matrices from decellularized tissues, provided that the cellular material is completely removed from them with the maximum possible preservation of the structure and composition of the natural extracellular matrix. The present study investigated the mechanical, biochemical, and biological properties of decellularized porcine cartilage microparticles (DCMps) obtained by techniques, differing only in physical treatments, such as freeze-thaw cycling (Protocol 1), supercritical carbon dioxide fluid (Protocol 2) and ultrasound (Protocol 3). Full tissue decellularization was achieved, as confirmed by the histological analysis and DNA quantification, though all the resultant DCMps had reduced glycosaminoglycans (GAGs) and collagen. The elastic modulus of all DCMp samples was also significantly reduced. Most notably, DCMps prepared with Protocol 3 significantly outperformed other samples in viability and the chondroinduction of the human adipose-derived stem cells (hADSCs), with a higher GAG production per DNA content. A positive ECM staining for type II collagen was also detected only in cartilage-like structures based on ultrasound-treated DCMps. The biocompatibility of a xenogenic DCMps obtained with Protocol 3 has been confirmed for a 6-month implantation in the thigh muscle tissue of mature rats (n = 18). Overall, the results showed that the porcine cartilage microparticles decellularized by a combination of detergents, ultrasound and DNase could be a promising source of scaffolds for TEMPs for cartilage reconstruction.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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