S. pombe wtf drivers use dual transcriptional regulation and selective protein exclusion from spores to cause meiotic drive.

Autor: Nuckolls NL; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Nidamangala Srinivasa A; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.; Department of Cell Biology and Physiology, University of Kansas Medical Center, Kansas City, Kansas, United States of America., Mok AC; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.; University of Missouri-Kansas City, Kansas City, Missouri, United States of America., Helston RM; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Bravo Núñez MA; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Lange JJ; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Gallagher TJ; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Seidel CW; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Zanders SE; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.; Department of Cell Biology and Physiology, University of Kansas Medical Center, Kansas City, Kansas, United States of America.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2022 Dec 07; Vol. 18 (12), pp. e1009847. Date of Electronic Publication: 2022 Dec 07 (Print Publication: 2022).
DOI: 10.1371/journal.pgen.1009847
Abstrakt: Meiotic drivers bias gametogenesis to ensure their transmission into more than half the offspring of a heterozygote. In Schizosaccharomyces pombe, wtf meiotic drivers destroy the meiotic products (spores) that do not inherit the driver from a heterozygote, thereby reducing fertility. wtf drivers encode both a Wtfpoison protein and a Wtfantidote protein using alternative transcriptional start sites. Here, we analyze how the expression and localization of the Wtf proteins are regulated to achieve drive. We show that transcriptional timing and selective protein exclusion from developing spores ensure that all spores are exposed to Wtf4poison, but only the spores that inherit wtf4 receive a dose of Wtf4antidote sufficient for survival. In addition, we show that the Mei4 transcription factor, a master regulator of meiosis, controls the expression of the wtf4poison transcript. This transcriptional regulation, which includes the use of a critical meiotic transcription factor, likely complicates the universal suppression of wtf genes without concomitantly disrupting spore viability. We propose that these features contribute to the evolutionary success of the wtf drivers.
Competing Interests: I have read the journal’s policy and the authors of this manuscript have the following competing interests: NLN, MABN, SEZ: Inventor on patent application based on wtf meiotic drivers. Patent application serial 62/491,107. The other authors declare that no competing interests exist.
(Copyright: © 2022 Nuckolls et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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