"In vivo" and "in vitro" antimicrobial activity of Origanum vulgare essential oil and its two phenolic compounds on clinical isolates of Candida spp.

Autor: Stringaro A; National Center of Drug Research and Evaluation, Italian National Institute of Health, Viale Regina Elena, 299, 00161, Rome, Italy., Colone M; National Center of Drug Research and Evaluation, Italian National Institute of Health, Viale Regina Elena, 299, 00161, Rome, Italy., Cecchetti S; Microscopy Unit and Core Facilities, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy., Zeppetella E; Department of Public Health and Infectious Diseases 'Sapienza', University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy., Spadaro F; Microscopy Unit and Core Facilities, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy., Angiolella L; Department of Public Health and Infectious Diseases 'Sapienza', University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy. letizia.angiolella@uniroma1.it.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2022 Dec 07; Vol. 205 (1), pp. 15. Date of Electronic Publication: 2022 Dec 07.
DOI: 10.1007/s00203-022-03355-1
Abstrakt: A limited therapeutic arsenal is currently available against Candida infections that show high resistance to antifungal agents. For this reason, there is a great need to prioritize testing therapeutic agents for the treatment of candidiasis. The use of essential oils and their phytoconstituents has been emphasized as a new therapeutic approach. The cell surface hydrophobicity (CSH), polysaccharide content, antimicrobial activity of essential oil from Origanum vulgare L. (OVEO), and its two phenolic compounds carvacrol and thymol were evaluated in four different Candida spp. (Candida albicans and emerging non-albicans Candida (NAC) species, such as C. glabrata, C. tropicalis, and C. krusei). The results showed the differences between Candida species; for example, C. tropicalis revealed higher resistance than other strains to different natural molecule treatments. The ultrastructural variabilities in the biomembranes and cell walls of these Candida spp. might explain the different biological effects observed after OVEO, carvacrol and thymol treatments. Therefore, to study the biological effects of these natural compounds on Candida strains, the samples were observed by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Moreover, the release of cellular materials and their "in vivo" antimicrobial activity on infected G. mellonella larvae were evaluated. The novelty of this study is the demonstration that exists a close correlation between both structural architecture of cell walls and biomembranes' organization with cell fungal responses to essential oils treatments. Overall, these results suggest practical limits to the predictability.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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