| Autor: |
Savin N; NUST MISiS, Moscow, Russian Federation. nsavin99@mail.ru., Erofeev A; NUST MISiS, Moscow, Russian Federation. nsavin99@mail.ru.; Lomonosov Moscow State University, Moscow, Russian Federation., Kolmogorov V; NUST MISiS, Moscow, Russian Federation. nsavin99@mail.ru.; Lomonosov Moscow State University, Moscow, Russian Federation., Salikhov S; NUST MISiS, Moscow, Russian Federation. nsavin99@mail.ru., Efremov Y; Institute for Regenerative Medicine I. M. Sechenov, Moscow, Russian Federation., Timashev P; Institute for Regenerative Medicine I. M. Sechenov, Moscow, Russian Federation.; World-class Research Center 'Digital Biodesign and Personalized Healthcare', Moscow, Russian Federation.; Chemistry department Lomonosov Moscow State University, Moscow, Russian Federation., Grammatikova N; G. F. Gauze Research Institute for New Antibiotics, Moscow, Russian Federation., Levshin I; G. F. Gauze Research Institute for New Antibiotics, Moscow, Russian Federation., Edwards C; Imperial College London, London, UK., Korchev Y; Imperial College London, London, UK., Gorelkin P; NUST MISiS, Moscow, Russian Federation. nsavin99@mail.ru. |
| Abstrakt: |
Super-resolution microscopy is widely used in the development of novel antimicrobial testing in vitro . In the presented work, a scanning protocol was developed by the method of scanning ion-conducting microscopy (SICM), which makes it possible to study microorganisms without rigid fixation and in saline, obtaining an index map of nanosized structures. The effect of azole and echinocandins drugs on the morphology and mechanical properties of Candida parapsilosis yeast was studied. The findings are consistent with previously proposed drug mechanisms and reports that have examined antifungal agents using AFM, SEM, and TEM. We have shown that the SICM method is capable of scanning and detecting the nanomechanical properties of yeast non-invasively. |
