Hybridization-Driven Adsorption of Polyadenine DNA onto Gold Nanoparticles.

Autor:	Sokolov PA; St. Petersburg University, 7/9 Universitetskaya Emb., St. Petersburg199034, Russia., Ramasanoff RR; Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy Prospect V.O. 31, St. Petersburg199004, Russia., Gabrusenok PV; St. Petersburg University, 7/9 Universitetskaya Emb., St. Petersburg199034, Russia., Baryshev AV; St. Petersburg University, 7/9 Universitetskaya Emb., St. Petersburg199034, Russia., Kasyanenko NA; St. Petersburg University, 7/9 Universitetskaya Emb., St. Petersburg199034, Russia.
Jazyk:	angličtina
Zdroj:	Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2022 Dec 20; Vol. 38 (50), pp. 15776-15781. Date of Electronic Publication: 2022 Dec 06.
DOI:	10.1021/acs.langmuir.2c02668
Abstrakt:	The attachment of functional DNA to gold nanoparticles via polyadenine adsorption is a well-established technology. This approach was mainly viewed through the lens of changing the DNA charge in order to reduce the electrostatic barrier created by a similarly charged gold surface. However, altering the DNA charge results in the loss of its functionality. This work considers the adsorption process of polyadenines by force that artificially brings them closer to the surface. As a force source, we used the hybridization of a DNA strand carrying polyadenines with a complementary strand already attached to the surface. It was shown that the hybridization forces facilitated the adsorption of polyadenines. We believe that this approach is applicable in various areas where it is essential to preserve the functionality of DNA during conjugation with nanoparticles.
Databáze:	MEDLINE
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