Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion.

Autor:	Udyavara Nagaraj V; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary.; Hevesy György Ph.D. School of Chemistry, Eötvös Loránd University, BudapestH-1117, Hungary., Juhász T; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary., Quemé-Peña M; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary.; Hevesy György Ph.D. School of Chemistry, Eötvös Loránd University, BudapestH-1117, Hungary., Szigyártó IC; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary., Bogdán D; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary.; Department of Organic Chemistry, Faculty of Pharmacy, Semmelweis University, BudapestH-1092, Hungary., Wacha A; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary., Mihály J; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary., Románszki L; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary., Varga Z; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary., Andréasson J; Department of Chemistry and Chemical Engineering, Physical Chemistry, Chalmers University of Technology, GothenburgSE-412 96, Sweden., Mándity I; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary.; Department of Organic Chemistry, Faculty of Pharmacy, Semmelweis University, BudapestH-1092, Hungary., Beke-Somfai T; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, BudapestH-1117, Hungary.; Department of Chemistry and Chemical Engineering, Physical Chemistry, Chalmers University of Technology, GothenburgSE-412 96, Sweden.
Jazyk:	angličtina
Zdroj:	ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2022 Dec 21; Vol. 14 (50), pp. 55320-55331. Date of Electronic Publication: 2022 Dec 06.
DOI:	10.1021/acsami.2c11946
Abstrakt:	Self-assembled peptide nanostructures with stimuli-responsive features are promising as functional materials. Despite extensive research efforts, water-soluble supramolecular constructs that can interact with lipid membranes in a controllable way are still challenging to achieve. Here, we have employed a short membrane anchor protein motif ( GLFD ) and coupled it to a spiropyran photoswitch. Under physiological conditions, these conjugates assemble into ∼3.5 nm thick, foil-like peptide bilayer morphologies. Photoisomerization from the closed spiro ( SP ) form to the open merocyanine ( MC ) form of the photoswitch triggers rearrangements within the foils. This results in substantial changes in their membrane-binding properties, which also varies sensitively to lipid composition, ranging from reversible nanofoil reformation to stepwise membrane adsorption. The formed peptide layers in the assembly are also able to attach to various liposomes with different surface charges, enabling the fusion of their lipid bilayers. Here, SP- to- MC conversion can be used both to trigger and to modulate the liposome fusion efficiency.
Databáze:	MEDLINE
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