| Autor: |
Smidt M; Laboratory of Analytical Toxicology, Feevale University, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525080, Brazil.; National Institute of Science & Technology in Forensic Sciences, Porto Alegre, Brazil.; Graduate Program on Toxicology & Analytical Toxicology, Feevale University, RS 239 2755, Novo Hamburgo, CEP 93525-075, Brazil., Bastiani MF; Laboratory of Analytical Toxicology, Feevale University, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525080, Brazil., Hahn RZ; Laboratory of Analytical Toxicology, Feevale University, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525080, Brazil., Lima Feltraco Lizot L; Laboratory of Analytical Toxicology, Feevale University, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525080, Brazil., Perassolo MS; Graduate Program on Toxicology & Analytical Toxicology, Feevale University, RS 239 2755, Novo Hamburgo, CEP 93525-075, Brazil., Linden R; Laboratory of Analytical Toxicology, Feevale University, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525080, Brazil.; National Institute of Science & Technology in Forensic Sciences, Porto Alegre, Brazil.; Graduate Program on Toxicology & Analytical Toxicology, Feevale University, RS 239 2755, Novo Hamburgo, CEP 93525-075, Brazil. |
| Abstrakt: |
Background: Dried blood spot sampling has been reported for on-site collection of specimens, but measurements are affected by blood hematocrit, and special handling is required, especially for forensic applications. The hemaPEN ® blood collection device was developed to produce spots with constant volume. Results: Linearity between 1 and 500 ng/ml was shown for cocaine and the metabolites benzoylecgonine and cocaethylene. The assay demonstrated acceptable precision and accuracy, and analytes were stable for 7 days when kept inside hemaPEN devices. Accuracy of the assay was affected by hematocrit but was within acceptable limits. Conclusion: Use of the hemaPEN, which retains dried blood within the device, could be advantageous for the quantification of illicit drugs in capillary blood compared with conventional dried blood spot collection. |
