Metabolic dysfunctions promoted by AIN-93G standard diet compared with three obesity-inducing diets in C57BL/6J mice.

Autor:	Aguiar LM; University of Campinas, School of Food Engineering, Department of Food Science and Nutrition, Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil., Moura CS; University of Campinas, School of Food Engineering, Department of Food Science and Nutrition, Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil., Ballard CR; University of Campinas, School of Food Engineering, Department of Food Science and Nutrition, Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil., Roquetto AR; University of Campinas, School of Food Engineering, Department of Food Science and Nutrition, Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil., Silva Maia JKD; University of Campinas, School of Food Engineering, Department of Food Science and Nutrition, Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil.; Federal University of Rio Grande Do Norte, Center for Health Sciences, Department of Nutrition, Av. Senador Salgado Filho 3000, Lagoa Nova, Natal, RN, Brazil., Duarte GHB; University of Campinas, Institute of Chemistry, Rua Josué de Castro, S/n - Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil., Costa LBED; University of Campinas, School of Medical Science, Rua Tessália Vieira de Camargo, 126 - Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil., Torsoni AS; University of Campinas, School of Applied Sciences, Rua Pedro Zaccaria, 1300, Limeira, SP, Brazil., Amaya-Farfan J; University of Campinas, School of Food Engineering, Department of Food Science and Nutrition, Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil., Maróstica Junior MR; University of Campinas, School of Food Engineering, Department of Food Science and Nutrition, Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil., Cazarin CBB; University of Campinas, School of Food Engineering, Department of Food Science and Nutrition, Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, Brazil.
Jazyk:	angličtina
Zdroj:	Current research in physiology [Curr Res Physiol] 2022 Nov 23; Vol. 5, pp. 436-444. Date of Electronic Publication: 2022 Nov 23 (Print Publication: 2022).
DOI:	10.1016/j.crphys.2022.11.001
Abstrakt:	Researchers from different fields have studied the causes of obesity and associated comorbidities, proposing ways to prevent and treat this condition by using a common animal model of obesity to create a profound energy imbalance in young adult rodents. However, to confirm the harmful effects of consuming a high-fat and hypercaloric diet, it is common to include normolipidic and normocaloric control groups in the experimental protocols. This study compared the effect of three experimental diets described in the literature - namely, a high-fat diet, a high-fat and high-sucrose diet, and a high-fat and high-fructose diet - to induce obesity in C57BL/6 J mice with the standard AIN-93G diet as a control. We hypothesize that the AIN diet formulation is not a good control in this type of experiment because this diet promotes weight gain and metabolic dysfunctions similar to the hypercaloric diet. The metabolic data of animals fed the AIN-93G diet were similar to those of the high-calorie groups (development of steatosis and hyperlipidemia). However, it is important to emphasize that the group fed a high-fat diet had a higher percentage of total fat (p = 0.0002) and abdominal fat (p = 0.013) compared to the other groups. Also, the high-fat group responded poorly to glucose and insulin tolerance tests, showing a picture of insulin resistance. As expected, the intake of the AIN-93G diet promotes metabolic alterations in the animals like the high-fat formulations. Therefore, although this diet continues to be used as the gold standard for growth and maintenance, it warrants a reassessment of its composition to minimize the metabolic changes observed in this study, thus updating its fitness as a normocaloric model of a standard rodent diet. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2022 The Authors.)
Databáze:	MEDLINE
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