Epirubicin may enhance the inhibition of hepatocellular carcinoma induced by iodine-125 seeds through downregulating WNT pathway.

Autor: Tian S; Department of Interventional Medicine, Shandong Cancer Hospital and Institute, Shandong First Medical University, Shandong Academy of Medical Sciences, Jinan, China., Lu Y; Cheeloo College of Medicine, Shandong University, Jinan, China., Gao H; Department of Oncology, Dongying People's Hospital, Dongying, China., Chen Z; Cheeloo College of Medicine, Shandong University, Jinan, China., Niu M; Department of Gastrointestinal Surgery, Traditional Chinese Medicine Hospital of Jiyang County, Jinan, China., Wang C; Department of Radiology, People's Hospital of Jiyang County, Jinan, China., Liu B; Department of Interventional Medicine, The Second Hospital of Shandong University, Jinan, China.; Interventional Oncology Institute of Shandong University, Jinan, China.
Jazyk: angličtina
Zdroj: Asia-Pacific journal of clinical oncology [Asia Pac J Clin Oncol] 2023 Jun; Vol. 19 (3), pp. 355-364. Date of Electronic Publication: 2022 Dec 04.
DOI: 10.1111/ajco.13873
Abstrakt: Aim: To investigate the role of iodine-125 ( 125 I) combined with epirubicin (EPI) in inhibiting hepatocellular carcinoma (HCC) growth and promoting apoptosis.
Methods: Both in vivo and in vitro experiments were conducted. CCK-8 assay was performed to determine the cells viability after EPI treatment. HepG2 and SMMC7721 cells were treated with EPI or 125 I or in combination. Colony formation assays were performed to verify the antiproliferation effect. Annexin V-FITC/PI, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, and western blotting were performed to analyze cellular apoptosis. Scratch wound healing assays and transwell assays were used to examine migration following different treatments. An isobaric tag for relative and absolute quantitation analysis was used to detect changes in protein expression after 125 I treatment, identifying the potential mediating protein cathelicidin (LL-37). LL-37 protein and WNT pathway-related proteins were detected by western blotting in SMMC7721 and HepG2 cells. Mice were treated with 125 I and EPI to evaluate whether EPI enhanced the antitumor effect of 125 I.
Results: EPI promoted 125 I-induced apoptosis and reduced the proliferation of HepG2 and SMMC7721 cells. EPI also prevented the migration of HepG2 and SMMC7721 cells. EPI combined with 125 I may interfere with the WNT signaling pathway by decreasing LL-37 to inhibit HCC development. The antitumor effects of EPI with 125 I were verified in mice.
Conclusion: EPI combined with 125 I induced apoptosis and inhibited the proliferation of HCC cells by LL-37 downregulating the WNT pathway.
(© 2022 The Authors. Asia-Pacific Journal of Clinical Oncology published by John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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