Evaluation of cytotoxicity and genotoxicity of a novel oxovanadium complex with orotate.

Autor: da Silva Gomes PS; UNIARA - University of Araraquara, Department of Biological Sciences and Health, CEP 14801-340, Araraquara, São Paulo, Brazil., da Silva WW; UNIARA - University of Araraquara, Department of Biological Sciences and Health, CEP 14801-340, Araraquara, São Paulo, Brazil., de Cássia Gasparoti G; UNIARA - University of Araraquara, Department of Biological Sciences and Health, CEP 14801-340, Araraquara, São Paulo, Brazil., Payolla FB; UNIARA - University of Araraquara, Department of Biological Sciences and Health, CEP 14801-340, Araraquara, São Paulo, Brazil., de Oliveira JA; UNESP - São Paulo State University, Faculty of Pharmaceutical Sciences of Araraquara, Department of Natural Active Principles and Toxicology, CEP 14801-902 Araraquara, São Paulo, Brazil., Barbugli PA; UNESP - São Paulo State University, School of Dentistry of Araraquara, CEP 14801-903, Araraquara, São Paulo, Brazil., Marin-Dett FH; UNESP - São Paulo State University, School of Dentistry of Araraquara, CEP 14801-903, Araraquara, São Paulo, Brazil., Cavicchioli M; UNIARA - University of Araraquara, Department of Biological Sciences and Health, CEP 14801-340, Araraquara, São Paulo, Brazil., Massabni AC; UNIARA - University of Araraquara, Department of Biological Sciences and Health, CEP 14801-340, Araraquara, São Paulo, Brazil., Resende FA; UNIARA - University of Araraquara, Department of Biological Sciences and Health, CEP 14801-340, Araraquara, São Paulo, Brazil. Electronic address: flaviabiomed@yahoo.com.br.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2022 Nov-Dec; Vol. 883-884, pp. 503558. Date of Electronic Publication: 2022 Oct 27.
DOI: 10.1016/j.mrgentox.2022.503558
Abstrakt: The development of new drugs based on metal complexes requires a detailed analysis of their biological endpoints. In this study, we report the genotoxic profile and influence on cell proliferation and death of the oxovanadium(IV) complex with orotic acid ([VO(C 5 H 4 N 2 O 4 ) 2 ], VO(oro)). Human hepatocellular carcinoma cells (HepG2) were the most sensitive tumor cells to VO(oro), which interfered with the integrity of cell membranes and proliferative capacity in a dose-dependent manner, inducing cell death by apoptosis. Regarding genotoxicity, VO(oro) did not induce considerable levels of DNA damage in HepG2 cells (comet test) and gene mutations (Ames test). However, it caused a statistically significant increase in the frequency of micronuclei at the highest concentration tested (12.5 µmol.L -1 ), indicating aneuploidy and clastogenicity. The data presented here provide information on various biological aspects of the VO(oro) complex, which may allow the elucidation of its mechanism of action as a possible therapeutic agent.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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