A stable isotope assay with 13 C-labeled polyethylene to investigate plastic mineralization mediated by Rhodococcus ruber.

Autor: Goudriaan M; Department of Marine Microbiology and Biogeochemistry (MMB), Royal Netherlands Institute of Sea Research (NIOZ), 1797 SZ 't Horntje, the Netherlands. Electronic address: maaike.goudriaan@nioz.nl., Morales VH; Department of Marine Microbiology and Biogeochemistry (MMB), Royal Netherlands Institute of Sea Research (NIOZ), 1797 SZ 't Horntje, the Netherlands; Centro de Investigación Mariña, University of Vigo, Department of Ecology and Animal Biology, Biological Oceanography Group, 36319 Vigo, Spain., van der Meer MTJ; Department of Marine Microbiology and Biogeochemistry (MMB), Royal Netherlands Institute of Sea Research (NIOZ), 1797 SZ 't Horntje, the Netherlands., Mets A; Department of Marine Microbiology and Biogeochemistry (MMB), Royal Netherlands Institute of Sea Research (NIOZ), 1797 SZ 't Horntje, the Netherlands., Ndhlovu RT; Department of Marine Microbiology and Biogeochemistry (MMB), Royal Netherlands Institute of Sea Research (NIOZ), 1797 SZ 't Horntje, the Netherlands., van Heerwaarden J; Department of Marine Microbiology and Biogeochemistry (MMB), Royal Netherlands Institute of Sea Research (NIOZ), 1797 SZ 't Horntje, the Netherlands., Simon S; MARUM-Center for Marine Environmental Sciences, University of Bremen, 28334 Bremen, Germany., Heuer VB; MARUM-Center for Marine Environmental Sciences, University of Bremen, 28334 Bremen, Germany., Hinrichs KU; MARUM-Center for Marine Environmental Sciences, University of Bremen, 28334 Bremen, Germany., Niemann H; Department of Marine Microbiology and Biogeochemistry (MMB), Royal Netherlands Institute of Sea Research (NIOZ), 1797 SZ 't Horntje, the Netherlands; Department of Earth Sciences, Faculty of Geosciences, Utrecht University, 3584 CB Utrecht, the Netherlands; CAGE-Centre for Arctic Gas Hydrate, Environment and Climate, Department of Geosciences, UiT the Arctic University of Norway, 9037 Tromsø, Norway. Electronic address: helge.niemann@nioz.nl.
Jazyk: angličtina
Zdroj: Marine pollution bulletin [Mar Pollut Bull] 2023 Jan; Vol. 186, pp. 114369. Date of Electronic Publication: 2022 Nov 30.
DOI: 10.1016/j.marpolbul.2022.114369
Abstrakt: Methods that unambiguously prove microbial plastic degradation and allow for quantification of degradation rates are necessary to constrain the influence of microbial degradation on the marine plastic budget. We developed an assay based on stable isotope tracer techniques to determine microbial plastic mineralization rates in liquid medium on a lab scale. For the experiments, 13 C-labeled polyethylene ( 13 C-PE) particles (irradiated with UV-light to mimic exposure of floating plastic to sunlight) were incubated in liquid medium with Rhodococcus ruber as a model organism for proof of principle. The transfer of 13 C from 13 C-PE into the gaseous and dissolved CO 2 pools translated to microbially mediated mineralization rates of up to 1.2 % yr -1 of the added PE. After incubation, we also found highly 13 C-enriched membrane fatty acids of R. ruber including compounds involved in cellular stress responses. We demonstrated that isotope tracer techniques are a valuable tool to detect and quantify microbial plastic degradation.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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